AIMS: This study aimed to determine how electric field strength, pulse width and shape, and specific energy input relate to the effect of pulsed electric fields (PEF) on viability and membrane permeabilization in Candida humilis and Saccharomyces cer...
Single-cell characterization and perturbation of neurons provides knowledge critical to addressing fundamental neuroscience questions including the structure-function relationship and neuronal cell-type classification. Here we report a robot for effi...
Electrowetting-on-dielectric (EWD) digital microfluidic laboratory-on-a-chip platforms demonstrate excellent performance in automating labor-intensive protocols. When coupled with an on-chip electroporation capability, these systems hold promise for ...
The international journal of medical robotics + computer assisted surgery : MRCAS
32112493
BACKGROUND: To evaluate the feasibility and safety of a robot-guided irreversible electroporation (IRE) ablation system for the treatment of pancreatic head carcinoma.
Single-cell delivery platforms like microinjection and nanoprobe electroporation enable unparalleled control over cell manipulation tasks but are generally limited in throughput. Here, we present an automated single-cell electroporation system capabl...
Small (Weinheim an der Bergstrasse, Germany)
35315229
Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing ma...
Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics involves introducing biomolecular cargoes into cells. However, successful delivery is a function of multiple experimental factors requiring several rounds of ...
The primary purpose of cancer treatment with irreversible electroporation (IRE) is to maximize tumor damage and minimize surrounding healthy tissue damage. Finite element analysis is one of the popular ways to calculate electric field and cell kill p...
The safety and efficacy of irreversible electroporation (IRE) in tumor therapy has been validated over many years by clinical application. An in-depth study, however, is required to assess the degree of ablation during the clinical dissemination of t...