[Rinsho ketsueki] The Japanese journal of clinical hematology
Jan 1, 2020
Precision medicine in oncology uses genomic data to provide the right intervention in the right patients at the right time. For this purpose, next-generation sequencing (NGS) is an indispensable tool. However, further innovations are necessary, inclu...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2020
A standard strategy to discover somatic mutations in a cancer genome is to use next-generation sequencing (NGS) technologies to sequence the tumor tissue and its matched normal (commonly blood or adjacent normal tissue) for side-by-side comparison. H...
BACKGROUND: Molecular profiling has become essential for tumor risk stratification and treatment selection. However, cancer genome complexity and technical artifacts make identification of real variants a challenge. Currently, clinical laboratories r...
MOTIVATION: With the recent advances in DNA sequencing technologies, the study of the genetic composition of living organisms has become more accessible for researchers. Several advances have been achieved because of it, especially in the health scie...
Understanding genotype-phenotype relationship is fundamental in biology. With the benefit from next-generation sequencing and high-throughput phenotyping methodologies, there have been generated much genome and phenome data for Saccharomyces cerevisi...
Cancer and its surgical treatment are among the most important triggering events for persistent pain, but additional factors need to be present for the clinical manifestation, such as variants in pain-relevant genes. In a cohort of 140 women undergoi...
Mathematical biosciences and engineering : MBE
Sep 30, 2019
Copy number variations (CNVs) play an important role in many types of cancer. With the rapid development of next generation sequencing (NGS) techniques, many methods for detecting CNVs of a single sample have emerged: (i) require genome-wide data of ...
ATAC-seq has been widely adopted to identify accessible chromatin regions across the genome. However, current data analysis still utilizes approaches initially designed for ChIP-seq or DNase-seq, without considering the transposase digested DNA fragm...
MOTIVATION: With the development of high-throughput sequencing techniques for 16S-rRNA gene profiling, the analysis of microbial communities is becoming more and more attractive and reliable. Inferring the direct interaction network among microbial c...
European review for medical and pharmacological sciences
Sep 1, 2019
OBJECTIVE: While next generation sequencing (NGS) has become the technology of choice for clinical diagnostics, most genetic laboratories still use Sanger sequencing for orthogonal confirmation of NGS results. Previous studies have shown that when th...