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Semen Preservation

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Effect of Timing of Postovulatory Insemination Relative to Human Chorionic Gonadotropin/Buserelin Treatment With 1 Straw of Frozen-Thawed Semen on Mare Fertility.

Journal of equine veterinary science
The reproductive management of mares for frozen semen artificial insemination (AI) can be costly and labor intensive. Predicting the exact time of ovulation can be challenging even when ovulation-inducing drugs are used. The main objective of this re...

Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions.

Journal of equine veterinary science
During semen processing for cryopreservation, most seminal plasma is usually removed, and components with protective effects on sperm may be missing after thawing and within the female reproductive tract. The present study evaluated the effect of pos...

Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm.

Journal of equine veterinary science
Studies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane in...

Dietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm quality.

Theriogenology
The aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n = ...

Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen.

Theriogenology
Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evalu...

Controlled ice nucleation--Is it really needed for large-volume sperm cryopreservation?

Theriogenology
Controlled ice nucleation (CIN) is an integral stage of slow freezing process when relatively large volumes (usually 1 mL or larger) of biological samples in suspension are involved. Without it, a sample will supercool to way below its melting point ...

Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure.

Theriogenology
The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO2 NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. ...

Designing of an artificial neural network model to evaluate the association of three combined Y-specific microsatellite loci on the actual and predicted postthaw motility in crossbred bull semen.

Theriogenology
The freezing of bull semen significantly hamper the motility of sperm which reduces the conception rate in dairy cattle. The prediction of postthaw motility (PTM) before freezing will be useful to take the decision on discarding or freezing of the ge...

Determination of the cryoprotective effect of n-methylacetamide in rabbit semen.

Cryo letters
BACKGROUND: Amides are low molecular weight cryoprotectants. N-methylacetamide (MA) is one of the cryoprotectant agents in this group.

IGF-1 stabilizes goat sperm mitochondrial transmembrane potential and reduces dna fragmentation.

Cryo letters
BACKGROUND: Antioxidant present in sperm cells protects them from oxidative damage. However, sperm are more susceptible to peroxidative damages due to the loss of these enzymes during cryopreservation and their survival and fertility may be compromis...