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Single-Cell Analysis

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What machine learning can do for developmental biology.

Development (Cambridge, England)
Developmental biology has grown into a data intensive science with the development of high-throughput imaging and multi-omics approaches. Machine learning is a versatile set of techniques that can help make sense of these large datasets with minimal ...

Optimal tuning of weighted kNN- and diffusion-based methods for denoising single cell genomics data.

PLoS computational biology
The analysis of single-cell genomics data presents several statistical challenges, and extensive efforts have been made to produce methods for the analysis of this data that impute missing values, address sampling issues and quantify and correct for ...

Neuronal differentiation strategies: insights from single-cell sequencing and machine learning.

Development (Cambridge, England)
Neuronal replacement therapies rely on the differentiation of specific cell types from embryonic or induced pluripotent stem cells, or on the direct reprogramming of differentiated adult cells via the expression of transcription factors or signaling...

Transfer learning efficiently maps bone marrow cell types from mouse to human using single-cell RNA sequencing.

Communications biology
Biomedical research often involves conducting experiments on model organisms in the anticipation that the biology learnt will transfer to humans. Previous comparative studies of mouse and human tissues were limited by the use of bulk-cell material. H...

Image-based phenotyping of disaggregated cells using deep learning.

Communications biology
The ability to phenotype cells is fundamentally important in biological research and medicine. Current methods rely primarily on fluorescence labeling of specific markers. However, there are many situations where this approach is unavailable or undes...

A data-driven dimensionality-reduction algorithm for the exploration of patterns in biomedical data.

Nature biomedical engineering
Dimensionality reduction is widely used in the visualization, compression, exploration and classification of data. Yet a generally applicable solution remains unavailable. Here, we report an accurate and broadly applicable data-driven algorithm for d...

Ensemble learning models that predict surface protein abundance from single-cell multimodal omics data.

Methods (San Diego, Calif.)
Single-cell protein abundance is a fundamental type of information to characterize cell states. Due to high cost and technical barriers, however, direct quantification of proteins is difficult. Single-cell RNA sequencing (scRNA-seq) data, serving as ...

A Machine Learning-Assisted Nanoparticle-Printed Biochip for Real-Time Single Cancer Cell Analysis.

Advanced biosystems
Cancers are a complex conglomerate of heterogeneous cell populations with varying genotypes and phenotypes. The intercellular heterogeneity within the same tumor and intratumor heterogeneity within various tumors are the leading causes of resistance ...

Identification of stem cells from large cell populations with topological scoring.

Molecular omics
Machine learning and topological analysis methods are becoming increasingly used on various large-scale omics datasets. Modern high dimensional flow cytometry data sets share many features with other omics datasets like genomics and proteomics. For e...

Detecting Interactive Gene Groups for Single-Cell RNA-Seq Data Based on Co-Expression Network Analysis and Subgraph Learning.

Cells
High-throughput sequencing technologies have enabled the generation of single-cell RNA-seq (scRNA-seq) data, which explore both genetic heterogeneity and phenotypic variation between cells. Some methods have been proposed to detect the related genes ...