Bacterial Infection

Elucidating the link between NS-SNP-Induced protein conformational changes and drug resistance in Klebsiella pneumoniae.

The escalating drug-resistance of Klebsiella pneumoniae (K. pneumoniae), a leading cause of both community-acquired and nosocomial infections, poses a severe threat to global public health. While non-synonymous single nucleotide polymorphisms (NS-SNPs) serve as crucial molecular markers in microbial resistance studies, their role in inducing protein conformational changes to confer resistance in K...

Classification and detection of Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes using Fourier-transform near infrared spectroscopy coupled with machine learning.

This study was conducted to investigate the potential use of Fourier-transform near-infrared (FT-NIR) spectroscopy combined with machine learning (ML) algorithms to accurately identify three foodborne pathogens, including Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. Each bacterial strain (two per pathogen) was individually cultured and purified through sequential washing ...

Noncanonical genetic markers resolve the pre-GOE emergence of aerobic bacteria in Earth's history.

The transition from anaerobic to aerobic life was a pivotal adaptation in Earth's history, yet the timing and genomic drivers remain poorly resolved. ...

One-Step Design of Potent and Nonhemolytic Antimicrobial Peptides by Using a Database-Guided, Nonmachine Learning Approach.

The search for antibiotics is urgent because of the global antibiotic resistance problem. While a variety of strategies are actively sought, interest ...

Establishment of threshold of human gut microbes and risk assessment system for colorectal cancer.

BACKGROUND: Being involved in the occurrence of colorectal cancer (CRC), gut microbes are potential targets for early diagnosis of CRC. Defining the t...

Whole-genome sequencing and comparative genomics reveal antimicrobial potential and adaptive traits of Bacillus velezensis AM12.

The global rise of antimicrobial resistance has intensified the demand for novel antimicrobial agents with broad-spectrum efficacy and unique mechanis...

A novel hypothetical protein (SAUSA300_1684) confers excellent protection against multi-drug-resistant Staphylococcus aureus infection in the murine model.

Staphylococcus aureus is a leading cause of nosocomial infections, including sepsis, bacteraemia, pneumonia, and endocarditis, and continues to pose a...

Deconstruction by C. thermocellum-from microbe mediated to dynamic redistribution of cellulosomes.

Clostridium thermocellum is one of the most efficient microorganisms for the deconstruction of cellulosic biomass. To achieve this high level of cellu...

Ai derivation and exploration of antibiotic class spaces.

PURPOSE: The rapid evolution of antibiotic-resistant bacteria poses an urgent global health crisis. A key gap in current antibiotic discovery approach...

Machine learning-based typing of Clostridium botulinum group III by FT-IR spectroscopy.

This study aimed to investigate the utility of Fourier-Transform Infrared Spectroscopy (FT-IRS) for differentiating Clostridium botulinum (C. botulinu...

Future Prospects for Using Clinical Phenotypes in Tuberculosis Precision Medicine-An Approach for Clinical Management.

Developing shorter treatment regimens for tuberculosis requires careful characterization of the clinical phenotype, which is defined by patient charac...

Caries Microbiome: time to move from blame to balance.

BACKGROUND: Advances in next-generation sequencing(NGS) and multi-omics approaches reinforced the concept of functional diversity within biofilm commu...

Identification of molecular compounds targeting bacterial propionate metabolism with topological machine learning.

This study demonstrates the transformative potential of machine learning in drug discovery by integrating comparative protein and ligand analysis with...

Artificial intelligence in the diagnosis and prognosis of pediatric bacterial pneumonia: current advances and challenges.

PURPOSE OF REVIEW: The clinical presentation of pediatric bacterial pneumonia often overlaps with that of other respiratory conditions, posing conside...

AI-Based D-Amino Acid Substitution for Optimizing Antimicrobial Peptides to Treat Multidrug-Resistant Bacterial Infection.

D-amino acid substitution provides an effective strategy for optimizing antimicrobial peptides (AMPs) by enhancing their stability. However, the absen...

Machine learning reveals sequence and methylation determinants of SaCas9-PAM interactions in bacteria.

Cas9 nucleases defend bacteria against invading DNA and can be used with single guide RNAs (sgRNAs) as antimicrobials and genome-editing tools. Howeve...

Recent advances in gold and zinc oxide nanoparticles: Antibiofilm action, mechanisms beyond ROS generation, and in vivo efficacy.

Gold (Au) and zinc oxide (ZnO) nanoparticles (AuNPs/ZnONPs, respectively) are well-established antimicrobial nanomaterials with broad-spectrum activit...

Automated high-throughput microscopy screening unveiled new Listeria monocytogenes genes involved in cell infection.

To uncover novel genetic factors required for Listeria monocytogenes cell infection, we developed an automated high-throughput microscopy screening pi...

Characteristics of phosphorus-solubilizing bacteria and mediated phosphorus cycle in sediments of urban river-lake interfaces.

Urban river-lake interfaces are crucial areas for substance migration and transformation, and prone to algal blooms. However, the phosphorus (P) cycle...

Non-contact identification of opportunistic pathogens in mixed biofilm contaminations by hyperspectral imaging.

BACKGROUND: Biofilms are present on almost all surfaces in households, healthcare and medical equipment, foods, industrial and water supply systems, a...