Genetics

Circulating MicroRNA Signatures in Severe Alopecia Areata: Diagnostic Discrimination, Pathway Analysis, and Therapeutic Implications.

INTRODUCTION: Alopecia areata (AA) is an autoimmune disorder characterized by non-scarring hair loss due to immune dysregulation. Despite advances, its precise molecular mechanisms remain unclear. This study investigates plasma microRNA (miRNA) expression profiles in patients with AA to identify biological pathways influenced by miRNAs and potential therapeutic targets. METHODS: A total of 50 pati...

Critical Assessment of a Structure-Based Pipeline for Targeting the Long Noncoding RNA MALAT1.

Long noncoding RNAs (lncRNAs) are increasingly recognized as druggable targets due to their conserved secondary/tertiary structures and regulatory roles in disease. A prototypical example is the MALAT1 triple helix, whose stability supports transcript persistence and is implicated in oncogenesis. Here, we evaluate the ability of a structure-based drug discovery (SBDD) pipeline, integrating molecul...

Hypoxia-related and immune phenotype-related fusion model for non-invasive prognostication of hepatocellular carcinoma treated by TACE: a multicentre study.

BACKGROUND: Survival outcomes after transarterial chemoembolisation (TACE) vary in hepatocellular carcinoma (HCC) patients, and existing prognostic sc...

The ATF5-GPER1 axis drives female protection in hepatocellular carcinoma through dual tumor-suppressive and immune-modulatory mechanisms.

Hepatocellular carcinoma (HCC) exhibits marked sexual dimorphism, with females demonstrating superior survival, yet the underlying molecular mechanism...

Genomic evolution of SARS-CoV-2 delta variants pre- and post-omicron emergence using alignment-free machine learning models.

The SARS-CoV-2 Delta variant (B.1.617.2), initially classified as a variant of concern due to its enhanced transmissibility and vaccine-escape mutatio...

AbDist: a lightweight, distance-based model for antibody affinity prediction as an interpretable benchmark for machine learning models.

Many complex models for antibody affinity prediction have been developed and successfully deployed. Recent results for T-cell receptor epitope predict...

MuTemAPR: Enhance Multilocation Patches with Template-Based Neural Program Repair.

Automated program repair (APR) has been studied extensively in recent years. Existing approaches mainly generate single-position patches that fail to ...

Hyperspectral phenotyping reveals the genetic basis of grain quality in rice.

Rice (Oryza sativa) grain quality is an important breeding target, yet its genetic basis remains incompletely understood. In this study, we integrated...

EPI-CAT: Prediction of enhancer-promoter interactions based on cross-attention transformer.

BACKGROUND: Enhancer-promoter interactions (EPIs) play a central role in transcriptional regulation, yet it remains an open challenge to identify whic...

Identification and validation of NETs-associated biomarkers in osteoporosis with diabetes.

Osteoporosis and diabetes represent major global public health challenges. Neutrophil extracellular traps (NETs) serve as key components of the innate...

Thymic health and immunotherapy outcomes in patients with cancer.

Although immunotherapy has revolutionized cancer treatment, many patients still experience limited benefit, highlighting the urgent need for improved ...

Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions.

Engineering CRISPR enzymes for high fidelity often impairs cleavage activity. Meanwhile, a mechanistic understanding of why high-fidelity mutations re...

The interplay between Peptostreptococcus and Fusobacterium as novel signatures in colorectal cancer recurrence.

BACKGROUND: After resection surgery, recurrence occurs in more than 30% of colorectal cancer (CRC) patients. Previous studies have highlighted the rol...

Identification of hypoxia-related gene signatures and molecular subtypes in chronic rhinosinusitis with nasal polyps.

OBJECTIVES: Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a common and severe subtype of Chronic Rhinosinusitis (CRS), characterized by persist...

Elucidating the mechanism of plasticizers associated with atherosclerosis through network toxicology, bulk RNA sequencing, and single-cell RNA sequencing analysis.

PURPOSE: This study aims to screen and identify potential atherosclerosis (AS) biomarkers associated with common plasticizer exposure, providing a bas...

Precision harvest: path to genetically modified organism-free crops with CRISPR by 2035.

Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR) technology enable precise genetic modifications and produce gene...

dMMR prediction from colorectal cancer histopathology: Leveraging non-tumor and low-magnification regions.

BACKGROUND AND OBJECTIVE: Colorectal cancer is the second leading cause of cancer-related mortality worldwide, posing a substantial burden on healthca...

Manufacturing-aware generative models enable petascale synthesis of designed DNA.

Generative modeling offers a robust framework for designing functional DNA, RNA and protein sequences. However, physical synthesis of these sequences ...

BKDRP: a biological knowledge-driven approach for drug response prediction using multi-omics data in cancer cell lines.

BACKGROUND: Cancer heterogeneity results in patients with the same diagnosis responding differently to drugs, making treatments extremely challenging....

Metabolite-mediated plant immunity: From traditional defensors to artificial elicitors.

The accelerating global population growth and increasing environmental pressures driven by climate change have made food security an urgent scientific...