Genetics

The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability.

OBJECTIVE: Osteosarcoma (OS) is a highly aggressive malignant bone tumor. While ID1 plays a critical role in OS progression, the underlying mechanisms remain unclear. This study investigates the METTL3-IGF2BP3 axis-mediated N6-methyladenosine (m6A) modification in regulating ID1 mRNA stability and its functional implications in OS. METHODS: RNA-binding proteins (RBPs) associated with ID1 were pred...

RNA2seg: a generalist model for cell segmentation in image-based spatial transcriptomics.

Imaging-based spatial transcriptomics enables high-resolution spatial mapping of RNA species. A key challenge in imaging-based spatial transcriptomics is accurate cell segmentation to assign each RNA molecule to the right cell. Here, we present RNA2seg, a novel segmentation algorithm trained on over 4 million cells from MERFISH and CosMx datasets across seven organs using a teacher-student trainin...

Parameter-efficient fine-tuning enables scalable transfer of regulatory sequence models to novel contexts.

BACKGROUND: DNA sequence deep learning models can accurately predict epigenetic and transcriptional profiles, enabling analysis of gene regulation and...

Raman spectroscopy with machine-learning classification for the prediction of stereotactic radiotherapy induced treatment toxicity in high-risk localised prostate cancer.

Radiotherapy can lead to late-onset toxicity, to varying extents between individuals due to differences in radiosensitivity. Predicting which patients...

Childhood dyslexia risk elevated by heavy metal mixtures from e-waste: A machine learning-driven mixture modeling study.

Environmental heavy metal mixtures from informal e-waste recycling are potential neurotoxicants, but their link to developmental dyslexia remains uncl...

Identification of the biomarkers associated with lipid metabolism and endoplasmic reticulum stress in pediatric sepsis through bioinformatics analysis and quantitative reverse transcriptase PCR (qRT-PCR) assay.

BACKGROUND: Sepsis constitutes a critical clinical condition characterized by organ failure and systemic inflammation, especially affecting children. ...

Hemoglobin as a Molecular Glue: Toward Potent Inhibition of HbS Polymerization in Sickle Cell Disease.

Sickle cell disease (SCD), a monogenic disorder arising from a single point mutation in the β-globin gene, continues to pose a significant global heal...

From inhibition to regulation: serpins in health and disease.

This issue of the Biomedical Journal highlights regulatory mechanisms that shape aging, disease progression, and biological complexity across molecula...

Evolution of root nodule symbiosis via paleopolyploidy and modular pathway rewiring.

The evolution of root nodule symbiosis (RNS), a key innovation for plant nitrogen acquisition, has long been studied but lacks a mechanistic, gene-lev...

Three-dimensional phenotyping: Technological advances and applications in genomics-assisted crop breeding.

With rapid advancements in breeding technologies, phenomics, and artificial intelligence, crop breeding is progressively advancing toward an era of gr...

Rapid Detection of NDM-Producing Carbapenem-Resistant Escherichia coli Using MALDI-TOF MS Combined with Machine Learning Techniques.

BACKGROUND: Carbapenem-resistant Escherichia coli (CREC) producing New Delhi metallo-beta-lactamase (NDM) poses a substantial threat to global public ...

Dual Confinement-Enhanced Multiple Single Nucleotide Variant Detection at the Single-Particle Level.

The efficacy of multiple single nucleotide variants (SNVs) analysis is far from ideal due to the limitations in identification. This study introduced ...

Renovating Neural Networks With Viral-Mediated Gene Transfer From A Tissue Contacting Matrix Mimic.

Neurodegenerative diseases such as Huntington's Disease (HD) have a significant impact on healthcare accessibility and costs. A fatal genetic conditio...

Droplet Digital CRISPR for Nucleic Acid Detection.

Droplet digital (dd) clustered regularly interspaced short palindromic repeats (CRISPR) integrates the high sequence specificity of CRISPR-based nucle...

Organ preservation in rectal cancer: an endoscopist's perspective on current evidence and future directions.

Rectal cancer management has increasingly shifted toward organ-preserving strategies that aim to maintain oncologic control while preserving bowel, ur...

Unraveling heterogeneity in LUAD via multi-omics integration: molecular classification and therapeutic implications.

Lung adenocarcinoma (LUAD) displays significant morphological and molecular heterogeneity both within and between tumors. This heterogeneity, coupled ...

Multi-cohort, cross-species urinary proteomics reveals signatures of LRRK2 dysfunction in Parkinson's disease.

Pathogenic mutations in Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson's disease (PD) and often increase kinase a...

Construction of a risk model based on exosome-related genes predict clinical prognosis and therapeutic response and revealing TIMP1 as a promising target in colorectal cancer.

Various exosome-derived proteins have been reported to play essential roles in regulating colorectal cancer progression and affecting the prognosis of...

Radiomics-based gradient boosting model on contrast-enhanced MRI for non-invasive prediction of epidermal growth factor receptor expression and therapeutic response to EGFR-targeted antibody-drug conjugates in high-grade glioma organoid models.

BACKGROUND: Epidermal growth factor (EGF) and its receptor EGF(EGFR) play crucial roles in glioblastoma (GBM) prognosis. However, non-invasive assessm...

Prefrontal cortex-extracellular vesicle RNAs coupling in alcohol use disorder: towards non-invasive biomarkers and targeted therapeutics.

Alcohol use disorder (AUD) poses a substantial global health burden, with its molecular mechanism remaining elusive. This study integrated datasets (G...