Genetics

Calcineurin-Dependent Stress Adaptation Enables Caspofungin Heteroresistance Leading to Stable Resistance in Candida Glabrata.

Antifungal heteroresistance has emerged as a clinical challenge across diverse species. In Candida glabrata, however, it has rarely been reported. Here, we characterized heteroresistance in a multicenter collection of 156 C. glabrata isolates, revealing a 25% prevalence with caspofungin specificity and 37°C dependent phenotypes. Transcriptomic profiling of the heteroresistant subpopulation under e...

Unveiling tumor heterogeneity by single cell RNA-sequencing: from basic considerations to clinical applications.

Tumor heterogeneity-encompassing diverse cellular phenotypes, genomic alterations, and micro-environmental contexts-is a principal barrier to effective cancer therapy. Single-cell RNA sequencing (scRNA-seq) has transformed our ability to resolve this complexity by capturing transcriptomes at single-cell resolution. Here, we review the technical foundations required for high-quality scRNA-seq studi...

Simulating the Dedifferentiation Process of Thyroid Cancer: Insights from Mouse Models and Ultrasound Imaging.

RATIONALE AND OBJECTIVE: Anaplastic thyroid carcinoma (ATC) may arise from the progression of differentiated thyroid cancers, but the mechanisms are n...

Identifying coagulation-related biomarkers in steroid-induced osteonecrosis of the femoral head: implications for early diagnosis and treatment.

Steroid-induced osteonecrosis of the femoral head (SONFH) is closely associated with coagulation dysfunction, but its molecular mechanisms remain inco...

Machine learning approaches to identify genetic markers for goat climatic adaptation.

UNLABELLED: Whole-genome SNP data from 109 goats, including 55 indigenous Indian goats and 54 exotic goats, were analyzed to identify ancestry informa...

Pharmacogenomic characterization of a uric acid metabolism-related signature associated with prognosis and drug sensitivity in gastric cancer.

Gastric cancer (GC) remains a major cause of cancer-related mortality worldwide. Increasing evidence suggests that metabolic reprogramming contributes...

Pan-cancer analysis identifies immunoproteasome as the predominant survival-associated component of antigen processing machinery.

Neoantigens are critical targets for cancer immunotherapy, yet the relationship between experimentally validated neoantigen burden and antigen process...

Multi-cohort integration and machine learning identify CPVL as a novel oncogenic driver in gastric cancer.

BACKGROUND: Gastric cancer (GC) remains a leading cause of cancer-related mortality worldwide, and the prognosis of advanced GC remains poor. Systemat...

Spatiotemporal genomic analysis and risk assessment of the plasmids carrying blaOXA-48-like genes based on a large-scale international dataset.

BACKGROUND: The spread of OXA-48-like carbapenemases represents a major public health challenge. Although previous studies have investigated OXA-48-li...

Comparative genomics and reproductive mechanisms of fertility in cattle and camels: Implications for assisted reproduction and precision breeding.

Fertility limits productivity in cattle and camels. Bovine fertility genomics is advanced, but determinants of fertility in camels remain poorly defin...

Research on identification of key genes and immune-metabolic mechanisms in atrial fibrillation through integrated multi-cohort transcriptomic analysis and machine learning.

This study aimed to integrate multiple datasets for the identification of atrial fibrillation (AF)-related differentially expressed genes (DEGs), anal...

Integrative analysis of machine learning and single-cell sequencing identifies IGHG1 as a therapeutic target for epimedium in diabetes-gastric cancer comorbidity.

BACKGROUND: The clinical comorbidity of diabetes mellitus (DM) and gastric cancer (GC) presents a significant healthcare challenge, as these two condi...

Integrative network toxicology and single-cell analysis implicates a potential TGFBR1-centered fibroblast remodeling axis in Benzo[a]pyrene-related atrial fibrillation.

BACKGROUND: Benzo[a]pyrene (BaP), an air pollution-related polycyclic aromatic hydrocarbon, may intersect with molecular pathways relevant to atrial f...

PASSpedia: A Polyadenylation Site Database Across Different Species at Single-cell Resolution.

Polyadenylation site (PAS) selection plays important roles in gene expression regulation and function. RNA sequencing (RNA-seq) data derived from 3' t...

AI-assisted digital breast tomosynthesis as a decision-support tool for post-neoadjuvant surgical planning in breast cancer.

PURPOSE: Accurate assessment of residual disease after neoadjuvant chemotherapy (NAC) is essential for surgical planning and prevention of incomplete ...

ZHMolTopoRPI: A Commutative Algebra-Driven Deep Learning Framework for Robust RNA-Protein Interaction Prediction.

Accurate prediction of RNA-protein interactions (RPIs) is crucial for understanding post-transcriptional regulation. Most existing models depend on im...

Electrochemical and optical biosensors as transformative tools for multiplex detection of influenza viruses.

The influenza viruses (IVs) are major public health risk, causing immense seasonal infections, severe illness, and respiratory deaths yearly. Rapid, a...

Disulfidptosis-related signatures identify SPARC+/CYR61+ stromal cells mediating immunosuppression in gastric cancer.

BACKGROUND: Disulfidptosis is a novel form of glucose starvation-induced cell death, yet its prognostic implications in gastric cancer (GC) remain lar...

The Cancer Metallome Continuum: A Framework for Understanding Metal Dysregulation from Carcinogenesis to Therapeutic Resistance.

Metals and metalloids play essential roles in cancer biology, influencing redox balance, epigenetic regulation, immune responses, and cell death throu...

Functional genes with their expression and horizontal gene transfer drive microbial interactions in anammox systems: Critical review and potential applications.

The anaerobic ammonium oxidation (anammox) process, a low-carbon and energy-efficient biological nitrogen removal technology, is crucial for sustainab...