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Binding, Competitive

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[Immunochromatographic Test System for the Detection of T-2 Toxin].

Prikladnaia biokhimiia i mikrobiologiia
An immunochromatographic test system was developed for the detection of T-2 toxin (T2T), which is one of priority contaminants of cereals. The detection is based on the competition between T2T in the sample and the T2T-protein conjugate immobilized o...

A deep boosting based approach for capturing the sequence binding preferences of RNA-binding proteins from high-throughput CLIP-seq data.

Nucleic acids research
Characterizing the binding behaviors of RNA-binding proteins (RBPs) is important for understanding their functional roles in gene expression regulation. However, current high-throughput experimental methods for identifying RBP targets, such as CLIP-s...

iSEE: Interface structure, evolution, and energy-based machine learning predictor of binding affinity changes upon mutations.

Proteins
Quantitative evaluation of binding affinity changes upon mutations is crucial for protein engineering and drug design. Machine learning-based methods are gaining increasing momentum in this field. Due to the limited number of experimental data, using...

CiRCus: A Framework to Enable Classification of Complex High-Throughput Experiments.

Journal of proteome research
Despite the increasing use of high-throughput experiments in molecular biology, methods for evaluating and classifying the acquired results have not kept pace, requiring significant manual efforts to do so. Here, we present CiRCus, a framework to gen...

Single and competitive dye adsorption onto chitosan-based hybrid hydrogels using artificial neural network modeling.

Journal of colloid and interface science
Chitosan-based hybrid hydrogels such as chitosan hydrogel (CH), chitosan hydrogel with activated carbon (CH-AC), scaffold-chitosan hydrogel (SCH), scaffold-chitosan hydrogel with activated carbon (SCH-AC) and scaffold-chitosan hydrogel with carbon na...

An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement.

Molecules (Basel, Switzerland)
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research. NanoLuc-based bioluminescence resonance energy transfer (Na...

Persistent spectral hypergraph based machine learning (PSH-ML) for protein-ligand binding affinity prediction.

Briefings in bioinformatics
Molecular descriptors are essential to not only quantitative structure activity/property relationship (QSAR/QSPR) models, but also machine learning based chemical and biological data analysis. In this paper, we propose persistent spectral hypergraph ...