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Chromatography, Affinity

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A Fluorescent Immunochromatography Test Strip for the Rapid Identification of SVV and FMDV.

Transboundary and emerging diseases
Seneca Valley virus (SVV) and foot-and-mouth disease virus (FMDV) belong to the Picornaviridae family, which can cause similar symptoms. After infection, pigs will develop fever; loss of appetite; blister lesions on the skin and mucous membrane of th...

[Evaluation of the lateral flow Aspergillus assay for the diagnosis of invasive aspergillosis, experience in a university hospital].

Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia
BACKGROUND: The incidence of invasive aspergillosis is increasing. Its diagnosis is based on clinical and microbiological criteria which include the determination of serological markers such as galactomannan. Recently, the Aspergillus lateral flow de...

Aptamer facilitated purification of functional proteins.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
DNA aptamers are attractive capture probes for affinity chromatography since, in contrast to antibodies, they can be chemically synthesized and, in contrast to tag-specific capture probes (such as Nickel-NTA or Glutathione), they can be used for puri...

Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen.

Talanta
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative ...

[Immunochromatographic Test System for the Detection of T-2 Toxin].

Prikladnaia biokhimiia i mikrobiologiia
An immunochromatographic test system was developed for the detection of T-2 toxin (T2T), which is one of priority contaminants of cereals. The detection is based on the competition between T2T in the sample and the T2T-protein conjugate immobilized o...

A competitive immunoassay for ultrasensitive detection of Hg(2+) in water, human serum and urine samples using immunochromatographic test based on surface-enhanced Raman scattering.

Analytica chimica acta
An immunochromatographic test (ICT) strip was developed for ultrasensitive competitive immunoassay of Hg(2+). This strategy was achieved by combining the easy-operation and rapidity of ICT with the high sensitivity of surface-enhanced Raman scatterin...

Robotic high-throughput purification of affinity-tagged recombinant proteins.

Methods in molecular biology (Clifton, N.J.)
Affinity purification of recombinant proteins has become the method of choice to obtain good quantities and qualities of proteins for a variety of downstream biochemical applications. While manual or FPLC-assisted purification techniques are generall...

A robust robotic high-throughput antibody purification platform.

Journal of chromatography. A
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...

Computer-aided design space identification for screening of protein A affinity chromatography resins.

Journal of chromatography. A
The rapidly growing market of monoclonal antibodies (mAbs) within the biopharmaceutical industry has incentivised numerous works on the design of more efficient production processes. Protein A affinity chromatography is regarded as one of the best pr...

A smartphone-integrated deep learning strategy-assisted rapid detection system for monitoring dual-modal immunochromatographic assay.

Talanta
This study focuses on the integration of a custom-built and optimally trained YOLO v5 model into a smartphone app developed with Java language. A dual-modal immunochromatographic rapid detection system based on a deep learning strategy for smartphone...