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Luciferases

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The effect of thalidomide on the invasive ability of gastric cancer cells by regulating miR-524-5p/FSTL1.

Cellular and molecular biology (Noisy-le-Grand, France)
This study aimed to investigate the effect of thalidomide (Thal) regulating microRNA (miR)-524-5p/follistatin-like protein 1 (FSTL1) on the invasion ability of gastric cancer cells. For this purpose, real-time fluorescent quantitative PCR (RT-qPCR) w...

Universal simultaneous multiplex ELISA of small molecules in milk based on dual luciferases.

Analytica chimica acta
The enzyme-linked immunosorbent assay (ELISA) has become the most important and widely used rapid detection technology for food safety because of its simple operation, fast speed and high sensitivity. Multiplex synchronous detection is the goal of EL...

Synthesis of Water-Soluble Imidazolium Polyesters as Potential Nonviral Gene Delivery Vehicles.

Biomacromolecules
The inherent hydrolytic reactivity of polyesters renders them excellent candidates for a variety of biomedical applications. Incorporating ionic groups further expands their potential impact, encompassing charge-dependent function such as deoxyribonu...

Preparation of Concentrated Chitosan/DNA Nanoparticle Formulations by Lyophilization for Gene Delivery at Clinically Relevant Dosages.

Journal of pharmaceutical sciences
Chitosan/DNA polyplexes have been optimized for efficient and safe in vitro and in vivo gene delivery. Clinical application of this technology requires the development of formulations with higher concentrations to reach therapeutic dosages. Polyplexe...

An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement.

Molecules (Basel, Switzerland)
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research. NanoLuc-based bioluminescence resonance energy transfer (Na...

De novo design of luciferases using deep learning.

Nature
De novo enzyme design has sought to introduce active sites and substrate-binding pockets that are predicted to catalyse a reaction of interest into geometrically compatible native scaffolds, but has been limited by a lack of suitable protein structur...

Electrically Driven, Bioluminescent Compliant Devices for Soft Robotics.

ACS applied materials & interfaces
Soft robotics, a research field wherein robots are fabricated from compliant materials, has sparked widespread research interest because of its potential applications in a variety of scenarios. In soft robots, luminescence is an important functionali...