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Chromatography, High Pressure Liquid

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A robust robotic high-throughput antibody purification platform.

Journal of chromatography. A
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...

Five-descriptor model to predict the chromatographic sequence of natural compounds.

Journal of separation science
Despite the recent introduction of mass detection techniques, ultraviolet detection is still widely applied in the field of the chromatographic analysis of natural medicines. Here, a neural network cascade model consisting of nine small artificial ne...

A Systematic Strategy for Screening and Application of Specific Biomarkers in Hepatotoxicity Using Metabolomics Combined With ROC Curves and SVMs.

Toxicological sciences : an official journal of the Society of Toxicology
Current studies that evaluate toxicity based on metabolomics have primarily focused on the screening of biomarkers while largely neglecting further verification and biomarker applications. For this reason, we used drug-induced hepatotoxicity as an ex...

Simultaneous quantification of 5 main components of Psoralea corylifolia L. in rats' plasma by utilizing ultra high pressure liquid chromatography tandem mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Psoralea corylifolia L. has long been used in traditional Chinese medicine for treating and preventing many diseases. A group of flavonoid components are regarded as the active principals within the seeds. In this research, a rapid, accurate and sens...

A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study.

Journal of pharmaceutical and biomedical analysis
Buddlejasaponin IV (BS-IV), a natural triterpene saponin isolated from several herbal plants, has drawn a lot of attention for its anti-inflammatory, antinociceptive, antihyperlipidemia, and antitumor activities. In this study, a simple and sensitive...

Determination of residual dextran sulfate in protein products by SEC-HPLC.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Dextran sulfate is a polyanionic derivative of dextran, produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate with an average molecular weight of 8000Da can be added to the cell culture to inhibit binding of proteins to cel...

Preparation, Characterization, and Antitumor Activities of Miriplatin-Loaded Liposomes.

Journal of pharmaceutical sciences
Because of the insolubility of miriplatin in water, miriplatin and lipiodol suspension is the sole formulation of miriplatin approved in Japan to treat hepatocellular carcinoma by transcatheter arterial chemoembolization. Until now, there have been n...

Fit-for-purpose chromatographic method for the determination of amikacin in human plasma for the dosage control of patients.

Talanta
In this paper, a simple, rapid and sensitive method based on liquid chromatography with fluorimetric detection (HPLC-FLD) for the determination of amikacin (AMK) in human plasma is developed. Determination is performed by pre-column derivatization of...

Simultaneous determination of glucose, fructose, sucrose and sorbitol in the leaf and fruit peel of different apple cultivars by the HPLC-RI optimized method.

Food chemistry
A high performance liquid chromatography method with refractive index detection (HPLC-RI), for simultaneous determination of glucose, fructose, sucrose and sorbitol in leaf and/or apple peel samples from nine apple (Malus domestica Borkh.) cultivars ...