AIMC Topic: Escherichia coli

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Design of Specific Peptide Inhibitors of Toxin-Antitoxin-Mediated Antiphage Defense.

ACS synthetic biology
Toxin-antitoxin (TA) systems are widespread antiphage defense elements in bacteria that may impede successful phage therapy. Phage-encoded inhibitors of these systems have been discovered that enhance phage infection capacity. We used fragment-based ...

Analysis of disruptive action of electrical current on cell membrane integrity and modelling its antimicrobial activity.

Archives of microbiology
It is essential to eliminate harmful microbes from vital aspects of our lives, including dental instruments and other healthcare devices, cosmetics, foods, and products that come into contact with them. Electrical stimulation (ES) has been proposed t...

Machine Learning-Assisted Terahertz Metagrating Biosensor for Label-Free Bacterial Identification Based on Spectral Fingerprinting.

Analytical chemistry
Rapid and accurate identification of pathogenic bacteria is pivotal for enabling early clinical diagnosis and precision antimicrobial therapy. In this study, we developed a label-free bacterial detection platform that integrates terahertz (THz) metag...

Unravelling mutation patterns in Extended-Spectrum β-Lactamases for precision drug design against AMR in Enterobacteriaceae.

Molecular genetics and genomics : MGG
Antimicrobial resistance (AMR) presents a critical global challenge, causing over 1.27 million deaths annually, with projections reaching 10 million by 2050. Among the most concerning contributors are Enterobacteriaceae, particularly Escherichia coli...

Evolutionary selection of trimethoprim-resistant genes in lytic phages affects phage and host fitness during infection.

Science advances
Temperate phages and prophages are well-known carriers of antibiotic resistance genes (ARGs) facilitating their transmission. In contrast, lytic phages rarely harbor functional ARGs. However, by a lenient threshold search strategy combined with a mac...

Novel antimicrobial peptide HFIAP-1 mutant as a β-lactamase inhibitor against extended-spectrum β-lactamases of Escherichia coli: a comprehensive in-silico approach.

Archives of microbiology
Extended-spectrum β-lactamases in Escherichia coli poses a significant threat for clinicians in tertiary healthcare settings, rendering treatments ineffective with newer β-lactam-β-lactamase inhibitors combinations. To overcome this, the present stud...

Employing deep mutational scanning in the periplasm to decode the thermodynamic landscape for amyloid formation.

Proceedings of the National Academy of Sciences of the United States of America
Deep mutational scanning (DMS) assays provide a powerful method to generate large-scale datasets essential for advancing AI-driven predictions in biology. The tripartite β-lactamase assay (TPBLA), in which a protein of interest is inserted between tw...

Bioinspired Multifunctional Eutectogels for Skin-Like Flexible Strain Sensors with Potential Application in Deep-Learning Handwriting Recognition.

Langmuir : the ACS journal of surfaces and colloids
Polymerizable deep eutectic solvents (PDES) have recently emerged as a class of solvent-free ionically conductive elastomers and are considered among the most feasible candidates for next-generation ionotronic devices. However, the fundamental challe...

Rapid label-free identification of seven bacterial species using microfluidics, single-cell time-lapse phase-contrast microscopy, and deep learning-based image and video classification.

PloS one
For effective treatment of bacterial infections, it is essential to identify the species causing the infection as early as possible. Current methods typically require hours of overnight culturing of a bacterial sample and a larger quantity of cells t...

Intelligent Design of Terminators by Coupling Prediction and Generation Models.

ACS synthetic biology
Terminators are specific nucleotide sequences located at the 3' end of a gene and contain transcription termination information. As a fundamental genetic regulatory element, terminators play a crucial role in the design of gene circuits. Accurately c...