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Escherichia coli

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Cost-effective batch production process of scFv antibody in Escherichia coli.

Human antibodies
BACKGROUND: Cost-effective production of antibody (Ab) fragments is of great interests of many pharmaceutical industries, in large part due to their high usages in research, diagnosis and therapy. Thus, the production of Abs necessitates accomplishme...

Medium Optimization for Recombinant Soluble Arginine Deiminase Expression in Escherichia coli Using Response Surface Methodology.

Current pharmaceutical biotechnology
OBJECTIVE: Optimization of the medium for recombinant arginine deiminase production in E. coli was performed using response surface methodology. This is the first study of optimization of recombinant arginine deiminase production in E. coli by the us...

[Evaluation of drinking-water treatment by Lifestraw® and Ceramic-pot filters].

Revista de salud publica (Bogota, Colombia)
Objective To evaluate under laboratory conditions, the removal efficiency of turbidity and E. coli of two household water filters: LifeStraw® family (MF) and ceramic pot filter (CPF). Methods The two systems were operated over 6 months using two iden...

Automated analysis of food-borne pathogens using a novel microbial cell culture, sensing and classification system.

The Analyst
We hereby report the design and implementation of an Autonomous Microbial Cell Culture and Classification (AMC(3)) system for rapid detection of food pathogens. Traditional food testing methods require multistep procedures and long incubation period,...

Research on Acute Toxicity and Pharmacodynamics of Jinyulian Oral Solution.

Cell biochemistry and biophysics
A research on Jinyulian Oral Solution was conducted and the objectives were to discover its possible acute toxicity and antibacterial effects when used in vitro and in vivo. Regarding the acute toxicity test, Kunming mice were fed a maximum amount of...

[Cloning, expression, and purification of c-di-AMP metabolism-related genes from Porphyromonas gingivalis].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
OBJECTIVE: To clone, express, and purify cyclic diadenosine monophosphate (c-di-AMP) metabolism-related genes from Porphyromonas gingivalis (P. gingivalis) ATCC33277.

Protein-protein interaction site prediction in Homo sapiens and E. coli using an interaction-affinity based membership function in fuzzy SVM.

Journal of biosciences
Protein-protein interaction (PPI) site prediction aids to ascertain the interface residues that participate in interaction processes. Fuzzy support vector machine (F-SVM) is proposed as an effective method to solve this problem, and we have shown tha...

Semi-supervised prediction of gene regulatory networks using machine learning algorithms.

Journal of biosciences
Use of computational methods to predict gene regulatory networks (GRNs) from gene expression data is a challenging task. Many studies have been conducted using unsupervised methods to fulfill the task; however, such methods usually yield low predicti...