AIMC Topic: Molecular Diagnostic Techniques

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A colorimetric Loop-mediated isothermal amplification (LAMP) assay based on HRP-mimicking molecular beacon for the rapid detection of Vibrio parahaemolyticus.

Biosensors & bioelectronics
In the world wide, food poisoning accidents related to Vibrio spp. are on the rise, even numbers of food poisoning by other foodborne pathogens are decreasing. Therefore, the requirement of the rapid, sensitive and convenient detection method for V. ...

An interpretation algorithm for molecular diagnosis of bacterial vaginosis in a maternity hospital using machine learning: proof-of-concept study.

Diagnostic microbiology and infectious disease
Allplex Bacterial vaginosis assay (Seegene, South Korea) is a molecular test for bacterial vaginosis (BV). A machine learning algorithm was devised on 200 samples (BV = 23, non-BV = 177) converting 7 identified bacterial strains polymerase chain reac...

Artificial intelligence in clinical and genomic diagnostics.

Genome medicine
Artificial intelligence (AI) is the development of computer systems that are able to perform tasks that normally require human intelligence. Advances in AI software and hardware, especially deep learning algorithms and the graphics processing units (...

Rapid microarray-based assay for detection of pyrazinamide resistant Mycobacterium tuberculosis.

Diagnostic microbiology and infectious disease
Pyrazinamide (PZA) is a key antibiotic for the treatment of drug susceptible tuberculosis. PZA-resistance is mainly mediated by mutations in the pncA gene; however the current gold standard is a phenotypic drug susceptibility test requiring a well-ad...

A Nasal Brush-based Classifier of Asthma Identified by Machine Learning Analysis of Nasal RNA Sequence Data.

Scientific reports
Asthma is a common, under-diagnosed disease affecting all ages. We sought to identify a nasal brush-based classifier of mild/moderate asthma. 190 subjects with mild/moderate asthma and controls underwent nasal brushing and RNA sequencing of nasal sam...

A European multicenter study on the analytical performance of the VERIS HBV assay.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
BACKGROUND: Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostic...

Inexpensive method for the quantitative estimation of hepatitis C virus RNA in blood plasma for low-resource settings using ML-based image intensity analysis of RT-LAMP products.

Lab on a chip
Hepatitis C virus (HCV) infection is a severe public health problem affecting nearly 3% of the world population. Of those affected, approximately 80% develop chronic infections. Initiating treatment through HCV RNA testing remains challenging, especi...

Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies.

Frontiers in cellular and infection microbiology
Tuberculosis (TB) remains a global health challenge, with timely and accurate diagnosis being critical for effective disease management and control. Recent advancements in the field of TB diagnostics have focused on the identification and utilization...

The Evolution of Nucleic Acid-Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design.

Methods in molecular biology (Clifton, N.J.)
Nucleic acid tests (NATs) are considered as gold standard in molecular diagnosis. To meet the demand for onsite, point-of-care, specific and sensitive, trace and genotype detection of pathogens and pathogenic variants, various types of NATs have been...