AIMC Topic: Molecular Diagnostic Techniques
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Melioidosis molecular diagnostics: An update.
Virulence
Jun 14, 2025
Melioidosis, a fatal tropical disease, presents a wide array of clinical manifestations, including abscesses, pneumonia, septic shock, bacteraemia, osteomyelitis, septic arthritis, and skin infection. The Centers for Disease Control and Prevention (C...
Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study.
Frontiers in cellular and infection microbiology
Jan 16, 2025
BACKGROUND: Though droplet digital PCR (ddPCR) has emerged as a promising tool for early pathogen detection in bloodstream infections (BSIs), more studies are needed to support its clinical application widely due to different ddPCR platforms with dis...
A drop dispenser for simplifying on-farm detection of foodborne pathogens.
PloS one
Dec 31, 2024
Nucleic-acid biosensors have emerged as useful tools for on-farm detection of foodborne pathogens on fresh produce. Such tools are specifically designed to be user-friendly so that a producer can operate them with minimal training and in a few simple...
Artificial intelligence-driven quantification of antibiotic-resistant Bacteria in food by color-encoded multiplex hydrogel digital LAMP.
Food chemistry
Dec 4, 2024
Antibiotic-resistant bacteria pose considerable risks to global health, particularly through transmission in the food chain. Herein, we developed the artificial intelligence-driven quantification of antibiotic-resistant bacteria in food using a color...
A novel machine-learning aided platform for rapid detection of urine ESBLs and carbapenemases: URECA-LAMP.
Journal of clinical microbiology
Oct 24, 2024
Pathogenic gram-negative bacteria frequently carry genes encoding extended-spectrum beta-lactamases (ESBL) and/or carbapenemases. Of great concern are carbapenem resistant , , and . Despite the need for rapid AMR diagnostics globally, current molecu...
Construction of a molecular diagnostic system for neurogenic rosacea by combining transcriptome sequencing and machine learning.
BMC medical genomics
Sep 13, 2024
Patients with neurogenic rosacea (NR) frequently demonstrate pronounced neurological manifestations, often unresponsive to conventional therapeutic approaches. A molecular-level understanding and diagnosis of this patient cohort could significantly g...
High-Throughput and Integrated CRISPR/Cas12a-Based Molecular Diagnosis Using a Deep Learning Enabled Microfluidic System.
ACS nano
Aug 22, 2024
CRISPR/Cas-based molecular diagnosis demonstrates potent potential for sensitive and rapid pathogen detection, notably in SARS-CoV-2 diagnosis and mutation tracking. Yet, a major hurdle hindering widespread practical use is its restricted throughput,...
No longer stuck in the past: new advances in artificial intelligence and molecular assays for parasitology screening and diagnosis.
Current opinion in infectious diseases
Aug 12, 2024
PURPOSE OF REVIEW: Emerging technologies are revolutionizing parasitology diagnostics and challenging traditional methods reliant on microscopic analysis or serological confirmation, which are known for their limitations in sensitivity and specificit...
Deep Learning Enabled Universal Multiplexed Fluorescence Detection for Point-of-Care Applications.
ACS sensors
Jul 15, 2024
There is a significant demand for multiplexed fluorescence sensing and detection across a range of applications. Yet, the development of portable and compact multiplexable systems remains a substantial challenge. This difficulty largely stems from th...
Enhanced detection of Listeria monocytogenes using tetraethylenepentamine-functionalized magnetic nanoparticles and LAMP-CRISPR/Cas12a-based biosensor.
Analytica chimica acta
Oct 11, 2023
BACKGROUND: Listeria monocytogenes is a pathogenic bacterium that can lead to severe illnesses, especially among vulnerable populations. Therefore, the development of rapid and sensitive detection methods is vital to prevent and manage foodborne dise...
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