AIMC Topic: Flow Cytometry

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Single Cell Analysis of Stored Red Blood Cells Using Ultra-High Throughput Holographic Cytometry.

Cells
Holographic cytometry is introduced as an ultra-high throughput implementation of quantitative phase imaging of single cells flowing through parallel microfluidic channels. Here, the approach was applied for characterizing the morphology of individua...

Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning.

Archives of toxicology
The in vitro micronucleus assay is a globally significant method for DNA damage quantification used for regulatory compound safety testing in addition to inter-individual monitoring of environmental, lifestyle and occupational factors. However, it re...

Cell morphology-based machine learning models for human cell state classification.

NPJ systems biology and applications
Herein, we implement and access machine learning architectures to ascertain models that differentiate healthy from apoptotic cells using exclusively forward (FSC) and side (SSC) scatter flow cytometry information. To generate training data, colorecta...

Experimental evaluation of deep learning method in reticulocyte enumeration in peripheral blood.

International journal of laboratory hematology
INTRODUCTION: Reticulocytes (RET) are immature red blood cells, and RET enumeration in peripheral blood has important clinical value in diagnosis, treatment efficacy observation, and prognosis of anemic diseases. For RET enumeration, flow cytometric ...

The in vitro micronucleus assay using imaging flow cytometry and deep learning.

NPJ systems biology and applications
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity. The MN assay is performed in two variations: scoring MN in cyt...

Investigating heterogeneities of live mesenchymal stromal cells using AI-based label-free imaging.

Scientific reports
Mesenchymal stromal cells (MSCs) are multipotent cells that have great potential for regenerative medicine, tissue repair, and immunotherapy. Unfortunately, the outcomes of MSC-based research and therapies can be highly inconsistent and difficult to ...

JSOM: Jointly-evolving self-organizing maps for alignment of biological datasets and identification of related clusters.

PLoS computational biology
With the rapid advances of various single-cell technologies, an increasing number of single-cell datasets are being generated, and the computational tools for aligning the datasets which make subsequent integration or meta-analysis possible have beco...

Machine learning and augmented human intelligence use in histomorphology for haematolymphoid disorders.

Pathology
Advances in digital pathology have allowed a number of opportunities such as decision support using artificial intelligence (AI). The application of AI to digital pathology data shows promise as an aid for pathologists in the diagnosis of haematologi...

Deep learning-enabled imaging flow cytometry for high-speed Cryptosporidium and Giardia detection.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Imaging flow cytometry has become a popular technology for bioparticle image analysis because of its capability of capturing thousands of images per second. Nevertheless, the vast number of images generated by imaging flow cytometry imposes great cha...