AIMC Topic: Nucleic Acid Amplification Techniques

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Simple and Multiplexed Detection of Nucleic Acid Targets Based on Fluorescent Ring Patterns and Deep Learning Analysis.

ACS applied materials & interfaces Nov 16, 2023
Simple diagnostic tests for nucleic acid targets can provide great advantages for applications such as rapid pathogen detection. Here, we developed a membrane assay for multiplexed detection of nucleic acid targets based on the visualization of two-d...
Nucleic Acid Amplification Techniques Fluorescent Dyes Nucleic Acids Oligonucleotide Probes SARS-CoV-2 Deep Learning
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Enhanced detection of Listeria monocytogenes using tetraethylenepentamine-functionalized magnetic nanoparticles and LAMP-CRISPR/Cas12a-based biosensor.

Analytica chimica acta Oct 11, 2023
BACKGROUND: Listeria monocytogenes is a pathogenic bacterium that can lead to severe illnesses, especially among vulnerable populations. Therefore, the development of rapid and sensitive detection methods is vital to prevent and manage foodborne dise...
CRISPR-Cas Systems Nucleic Acid Amplification Techniques Biosensing Techniques Endodeoxyribonucleases Magnetite Nanoparticles Limit of Detection Molecular Diagnostic Techniques Bacterial Proteins CRISPR-Associated Proteins Listeria monocytogenes Food Microbiology
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Programmable magnetic robot (ProMagBot) for automated nucleic acid extraction at the point of need.

Lab on a chip Aug 22, 2023
Upstream sample preparation remains the bottleneck for point-of-need nucleic acid testing due to its complexity and time-consuming nature. Sample preparation involves extracting, purifying, and concentrating nucleic acids from various matrices. These...
Nucleic Acid Amplification Techniques Robotics Magnetic Phenomena Nucleic Acids Magnetics
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Paper microfluidics with deep learning for portable intelligent nucleic acid amplification tests.

Talanta Mar 20, 2023
During global outbreaks such as COVID-19, regular nucleic acid amplification tests (NAATs) have posed unprecedented burden on hospital resources. Data of traditional NAATs are manually analyzed post assay. Integration of artificial intelligence (AI) ...
Deep Learning Nucleic Acid Amplification Techniques Microfluidics Humans SARS-CoV-2 Sensitivity and Specificity Artificial Intelligence COVID-19
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An automated, fully-integrated nucleic acid analyzer based on microfluidic liquid handling robot technique.

Analytica chimica acta Dec 1, 2022
On-site nucleic acid testing (NAT) plays an important role for disease monitoring and pathogen diagnosis. In this work, we developed an automated and fully-integrated nucleic acid analyzer by combining the automated liquid handling robot technique wi...
Nucleic Acids Microfluidic Analytical Techniques Real-Time Polymerase Chain Reaction Nucleic Acid Amplification Techniques Robotics Humans Microfluidics
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Ferrobotic swarms enable accessible and adaptable automated viral testing.

Nature Nov 9, 2022
Expanding our global testing capacity is critical to preventing and containing pandemics. Accordingly, accessible and adaptable automated platforms that in decentralized settings perform nucleic acid amplification tests resource-efficiently are requi...
Pandemics COVID-19 Algorithms SARS-CoV-2 Humans Robotics RNA, Viral COVID-19 Testing Automation Sensitivity and Specificity Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques Magnets Indicators and Reagents
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Intelligent and robust DNA robots capable of swarming into leakless nonlinear amplification in response to a trigger.

Nanoscale horizons May 31, 2022
Nonlinear DNA signal amplification with an enzyme-free isothermal self-assembly process is uniquely useful in nanotechnology and nanomedicine. However, progress in this direction is hampered by the lack of effective design models of leak-resistant DN...
DNA Nanostructures Nucleic Acid Amplification Techniques Robotics Biosensing Techniques
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A deep learning-driven low-power, accurate, and portable platform for rapid detection of COVID-19 using reverse-transcription loop-mediated isothermal amplification.

Scientific reports Mar 8, 2022
This paper presents a deep learning-driven portable, accurate, low-cost, and easy-to-use device to perform Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) to facilitate rapid detection of COVID-19. The 3D-printed device-powered...
RNA, Viral Coloring Agents COVID-19 Testing Nasopharynx Nucleic Acid Amplification Techniques Printing, Three-Dimensional Area Under Curve Deep Learning ROC Curve Molecular Diagnostic Techniques Humans Sensitivity and Specificity COVID-19 Point-of-Care Systems SARS-CoV-2
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Loop-mediated isothermal amplification (LAMP) and machine learning application for early pregnancy detection using bovine vaginal mucosal membrane.

Biochemical and biophysical research communications Jul 10, 2021
An early and accurate pregnancy diagnosis method is required to improve the reproductive performance of cows. Here we developed an easy pregnancy detection method using vaginal mucosal membrane (VMM) with application of Reverse Transcription-Loop-med...
Pregnancy Female Reproducibility of Results Cattle RNA-Binding Proteins CA-125 Antigen Sensitivity and Specificity Ubiquitins Animals Machine Learning Cytokines Adaptor Proteins, Signal Transducing Biomarkers Molecular Diagnostic Techniques Gene Expression Mucous Membrane Membrane Proteins Vagina Nucleic Acid Amplification Techniques
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Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2.

Viruses Sep 1, 2020
Until vaccines and effective therapeutics become available, the practical solution to transit safely out of the current coronavirus disease 19 (CoVID-19) lockdown may include the implementation of an effective testing, tracing and tracking system. Ho...
Pneumonia, Viral Vero Cells Clinical Laboratory Techniques Betacoronavirus Sensitivity and Specificity Madin Darby Canine Kidney Cells Animals Pandemics Nucleic Acid Amplification Techniques Humans Coronavirus Infections COVID-19 Real-Time Polymerase Chain Reaction COVID-19 Testing RNA, Viral Artificial Intelligence SARS-CoV-2 Molecular Diagnostic Techniques Chlorocebus aethiops Dogs
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