Infectious Disease

CbpM and CbpG of Streptococcus Pneumoniae Elicit a High Protection in Mice Challenged with a Serotype 19F Pneumococcus.

Among many pneumococcal antigens, choline-binding proteins (CPBs) display a high immunogenicity in animal models. This study aims to determine the immunogenicity of CbpM, CbpG and CbpL proteins of Streptococcus pneumoniae in a mice model. The genes were cloned into pET21a expression vector and the recombinant proteins were produced. Mice were immunized with the purified recombinant proteins. Subse...

Performance of convolutional neural networks for identification of bacteria in 3D microscopy datasets.

Three-dimensional microscopy is increasingly prevalent in biology due to the development of techniques such as multiphoton, spinning disk confocal, and light sheet fluorescence microscopies. These methods enable unprecedented studies of life at the microscale, but bring with them larger and more complex datasets. New image processing techniques are therefore called for to analyze the resulting ima...

Novel neural network application for bacterial colony classification.

BACKGROUND: Bacterial colony morphology is the first step of classifying the bacterial species before sending them to subsequent identification proces...

Prevalence and predictors of C. difficile infections in hospitalized patients with major surgical procedures in the USA: Analysis using traditional and machine learning methods.

Genotoxicity evaluation of titanium dioxide nanoparticles using the mouse lymphoma assay and the Ames test.

Titanium dioxide nanoparticles (TiO-NPs) are widely used in the cosmetics, health, and food industries, but their safety and genotoxicity remain a mat...

In vitro activity of tedizolid and other comparator drugs in methicillin-resistant Staphylococcus aureus isolates in skin and soft tissue infections in seven Colombian hospitals.

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) causes severe skin and soft tissue infections in hospitals and, more recently, in the...

Immunogenicity of Concentrated and Purified Inactivated Avian Influenza Vaccine Formulation.

Avian influenza (AI) H9N2 is a low pathogenic virus subtype belonging to Orthomyxoviridae family. Given the prevalence of this subtype as an infectiou...

Do reductions in agricultural field drainage during the growing season impact bacterial densities and loads in small tile-fed watersheds?

Predicting bacterial levels in watersheds in response to agricultural beneficial management practices (BMPs) requires understanding the germane proces...

Identification of Immune Signatures of Novel Adjuvant Formulations Using Machine Learning.

Adjuvants have long been critical components of vaccines, but the exact mechanisms of their action and precisely how they alter or enhance vaccine-ind...

Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning.

Streptococcus uberis is one of the most common pathogens of clinical mastitis in the dairy industry. Knowledge of pathogen transmission route is essen...

Design and Synthesis of Imidazo/Benzimidazo[1,2-]quinazoline Derivatives and Evaluation of Their Antimicrobial Activity.

A new class of fused quinazolines has been designed and synthesized via copper-catalyzed Ullmann type C-N coupling followed by intramolecular cross-de...

Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.

A colorimetric nucleic acid based test for label-free pathogen detection has been developed and used for the detection of the Zika virus. The test rel...

Antifungal, anti-oxidant activity and cytotoxicity of South African medicinal plants against mycotoxigenic fungi.

Fungal strains belonging to genus and are known to infect crops, resulting in threatened food security and less agricultural crop yields. The aim of...

Detection of herpesvirus capsids in transmission electron microscopy images using transfer learning.

The detailed analysis of secondary envelopment of the Human betaherpesvirus 5/human cytomegalovirus (HCMV) from transmission electron microscopy (TEM)...

The feasibility of fingerstick blood collection for point-of-care HIV-1 viral load monitoring in rural Zambia.

Viral load monitoring for HIV treatment is recommended but not feasible in many settings. A point-of-care test using capillary blood would increase ac...

In vitro activity of flomoxef against extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Korea.

To find an alternative regimen for the treatment of extended-spectrum β-lactamase (EBSL)-producing Enterobacteriaceae infections, we examined the in v...

Unusual presence of 'ghosts' following lead extraction for recurrent reactive pericarditis: a case report.

BACKGROUND: The presence of a persistent fibrous sheath in right-sided heart chambers after transvenous lead extraction has already been described in ...

Combination Therapy of Clinically Approved Antifungal Drugs Is Enhanced by Conjugation with Silver Nanoparticles.

Silver nanoparticles (SN) have been recently developed as a new class of antimicrobial agents against numerous pathogenic microorganisms. SN have also...

Level of circulating steroid hormones in malaria and cutaneous leishmaniasis: a case control study.

Epidemiological and clinical studies have shown a great difference in the severity and prevalence of infectious diseases in men and women and various ...

Rapid Rule Out of Culture-Negative Bloodstream Infections by Use of a Novel Approach to Universal Detection of Bacteria and Fungi.

BACKGROUND: Currently it can take up to 5 days to rule out bloodstream infection. With the low yield of blood cultures (approximately 10%), a signific...