AIMC Topic: Culture Media
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High-throughput transformation of Saccharomyces cerevisiae using liquid handling robots.
PloS one
Mar 20, 2017
Saccharomyces cerevisiae (budding yeast) is a powerful eukaryotic model organism ideally suited to high-throughput genetic analyses, which time and again has yielded insights that further our understanding of cell biology processes conserved in human...
Optimization of culture conditions for gamma-aminobutyric acid production in fermented adzuki bean milk.
Journal of food and drug analysis
Feb 13, 2017
γ-Aminobutyric acid (GABA), a nonprotein amino acid, is widely distributed in nature and fulfills several physiological functions. In this study, various lactic acid strains commonly used to produce fermented milk products were inoculated into adzuki...
Analytical Method for the Detection of Residual Active Ingredients Found in Neutralized Suspensions of Antimicrobial Products.
Journal of AOAC International
Nov 10, 2016
An analytical method for determining the presence and levels of residual active ingredients found in neutralized suspensions of phenolic and quaternary ammonium salt-based antimicrobial products was developed using solid-phase extraction in combinati...
In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption.
Theriogenology
Nov 9, 2016
The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicle...
MicrO: an ontology of phenotypic and metabolic characters, assays, and culture media found in prokaryotic taxonomic descriptions.
Journal of biomedical semantics
Apr 12, 2016
BACKGROUND: MicrO is an ontology of microbiological terms, including prokaryotic qualities and processes, material entities (such as cell components), chemical entities (such as microbiological culture media and medium ingredients), and assays. The o...
Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts.
BioMed research international
Dec 22, 2015
INTRODUCTION: Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation. Interleukin-1β is highly expressed during fracture healing and has been demonstrated to exert a significant...
Insulin improves in vitro survival of equine preantral follicles enclosed in ovarian tissue and reduces reactive oxygen species production after culture.
Theriogenology
Nov 24, 2015
This study investigated the effect of insulin concentration on the in vitro culture of equine preantral follicles enclosed in ovarian tissue. Ovarian tissue samples were immediately fixed (noncultured control) or cultured for 1 or 7 days in α-MEM(+) ...
Artificial neural network associated to UV/Vis spectroscopy for monitoring bioreactions in biopharmaceutical processes.
Bioprocess and biosystems engineering
Jan 1, 2015
Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process moni...
Machine learning assisted media optimization for enhanced insulin production in Pseudomonas fluorescens cell factory and scale-up studies.
International journal of biological macromolecules
Jun 1, 2025
Diabetes mellitus, a chronic metabolic disorder, is characterized by high blood glucose levels. External insulin administration, along with diet and exercise, is recommended to the patients. The increase in the number of diabetics and the adoption of...
Deep-learning-assisted medium optimization improves hyaluronic acid production by Streptococcus zooepidemicus.
Journal of bioscience and bioengineering
Jun 1, 2025
To improve the efficiency of hyaluronic acid production by Streptococcus zooepidemicus, the growth medium was optimized with a pipeline involving a deep learning (DL) algorithm. To train the DL model, the initial training dataset (OA01-18) was design...
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