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Culture Media

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Medium Optimization for Recombinant Soluble Arginine Deiminase Expression in Escherichia coli Using Response Surface Methodology.

Current pharmaceutical biotechnology
OBJECTIVE: Optimization of the medium for recombinant arginine deiminase production in E. coli was performed using response surface methodology. This is the first study of optimization of recombinant arginine deiminase production in E. coli by the us...

Optimizing light distribution and controlling biomass concentration by continuously pre-harvesting Spirulina platensis for improving the microalgae production.

Bioresource technology
To improve the microalgae production in batch cultivation, a cultivation mode that continuously pre-harvesting Spirulina platensis from photobioreactor (PBR) with culture medium recycling was proposed. For realizing the continuously pre-harvesting cu...

Production and characterization of a biosurfactant produced by Streptomyces sp. DPUA 1559 isolated from lichens of the Amazon region.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
Surfactants are amphipathic compounds containing both hydrophilic and hydrophobic groups, capable to lower the surface or interfacial tension. Considering the advantages of the use of biosurfactants produced by microorganisms, the aim of this paper w...

In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption.

Theriogenology
The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicle...

Analytical Method for the Detection of Residual Active Ingredients Found in Neutralized Suspensions of Antimicrobial Products.

Journal of AOAC International
An analytical method for determining the presence and levels of residual active ingredients found in neutralized suspensions of phenolic and quaternary ammonium salt-based antimicrobial products was developed using solid-phase extraction in combinati...

[Effects of Browning Inhibitors on Suspension Cells Growth and Secondary Metabolites Production in Changium smyrnioides].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
OBJECTIVE: To study the effects of browning inhibitors on Changium smyrnioides suspension cells growth and secondary metabolites production.

[Condition optimization for bio-oxidation of high-S and high-As gold concentrate].

Wei sheng wu xue bao = Acta microbiologica Sinica
OBJECTIVE: To study the effects of temperature and lixivium return on the concentrate bio-oxidation and rate of gold cyanide leaching.

[Enhancement of Coprinus cinereus peroxidase in Pichia pastoris by co-expression chaperone PDI and Ero1].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
The 1,095 bp gene encoding peroxidase from Coprinus cinereus was synthesized and integrated into the genome of Pichia pastoris with a highly inducible alcohol oxidase. The recombinant CiP (rCiP) fused with the a-mating factor per-pro leader sequence ...

MicrO: an ontology of phenotypic and metabolic characters, assays, and culture media found in prokaryotic taxonomic descriptions.

Journal of biomedical semantics
BACKGROUND: MicrO is an ontology of microbiological terms, including prokaryotic qualities and processes, material entities (such as cell components), chemical entities (such as microbiological culture media and medium ingredients), and assays. The o...

High-throughput transformation of Saccharomyces cerevisiae using liquid handling robots.

PloS one
Saccharomyces cerevisiae (budding yeast) is a powerful eukaryotic model organism ideally suited to high-throughput genetic analyses, which time and again has yielded insights that further our understanding of cell biology processes conserved in human...