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Tissue Culture Techniques

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Insulin improves in vitro survival of equine preantral follicles enclosed in ovarian tissue and reduces reactive oxygen species production after culture.

Theriogenology
This study investigated the effect of insulin concentration on the in vitro culture of equine preantral follicles enclosed in ovarian tissue. Ovarian tissue samples were immediately fixed (noncultured control) or cultured for 1 or 7 days in α-MEM(+) ...

An artificial intelligence approach for modeling volume and fresh weight of callus - A case study of cumin (Cuminum cyminum L.).

Journal of theoretical biology
Cumin (Cuminum cyminum Linn.) is valued for its aroma and its medicinal and therapeutic properties. A supervised feedforward artificial neural network (ANN) trained with back propagation algorithms, was applied to predict fresh weight and volume of C...

Artificial neural network-based model for the prediction of optimal growth and culture conditions for maximum biomass accumulation in multiple shoot cultures of Centella asiatica.

Protoplasma
An artificial neural network (ANN)-based modelling approach is used to determine the synergistic effect of five major components of growth medium (Mg, Cu, Zn, nitrate and sucrose) on improved in vitro biomass yield in multiple shoot cultures of Cente...

Dendritic spine classification using shape and appearance features based on two-photon microscopy.

Journal of neuroscience methods
BACKGROUND: Neuronal morphology and function are highly coupled. In particular, dendritic spine morphology is strongly governed by the incoming neuronal activity. The first step towards understanding the structure-function relationships is to classif...

Robotically handled whole-tissue culture system for the screening of oral drug formulations.

Nature biomedical engineering
Monolayers of cancer-derived cell lines are widely used in the modelling of the gastrointestinal (GI) absorption of drugs and in oral drug development. However, they do not generally predict drug absorption in vivo. Here, we report a robotically hand...

CytoCensus, mapping cell identity and division in tissues and organs using machine learning.

eLife
A major challenge in cell and developmental biology is the automated identification and quantitation of cells in complex multilayered tissues. We developed CytoCensus: an easily deployed implementation of supervised machine learning that extends conv...

Robust Phase Unwrapping via Deep Image Prior for Quantitative Phase Imaging.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society
Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase unwrapping is the ...

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection.

Journal of visualized experiments : JoVE
A central question in developmental neurobiology is how neural stem and progenitor cells form the brain. To answer this question, one needs to label, manipulate, and follow single cells in the brain tissue with high resolution over time. This task is...

A programmable, open-source robot that scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.

Cell reports methods
Despite the widespread popularity of the "scratch assay," where a pipette is dragged manually through cultured tissue to create a gap to study cell migration and healing, it carries significant drawbacks. Its heavy reliance on manual technique can co...