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Single-Cell Analysis

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Coupled co-clustering-based unsupervised transfer learning for the integrative analysis of single-cell genomic data.

Briefings in bioinformatics
Unsupervised methods, such as clustering methods, are essential to the analysis of single-cell genomic data. The most current clustering methods are designed for one data type only, such as single-cell RNA sequencing (scRNA-seq), single-cell ATAC seq...

Deep-joint-learning analysis model of single cell transcriptome and open chromatin accessibility data.

Briefings in bioinformatics
Simultaneous profiling transcriptomic and chromatin accessibility information in the same individual cells offers an unprecedented resolution to understand cell states. However, computationally effective methods for the integration of these inherent ...

scCancer: a package for automated processing of single-cell RNA-seq data in cancer.

Briefings in bioinformatics
Molecular heterogeneities and complex microenvironments bring great challenges for cancer diagnosis and treatment. Recent advances in single-cell RNA-sequencing (scRNA-seq) technology make it possible to study cancer cell heterogeneities and microenv...

Integration and transfer learning of single-cell transcriptomes via cFIT.

Proceedings of the National Academy of Sciences of the United States of America
Large, comprehensive collections of single-cell RNA sequencing (scRNA-seq) datasets have been generated that allow for the full transcriptional characterization of cell types across a wide variety of biological and clinical conditions. As new methods...

Identification of Gene Regulatory Networks from Single-Cell Expression Data.

Methods in molecular biology (Clifton, N.J.)
Single-cell RNAseq is an emerging technology that allows the quantification of gene expression in individual cells. In plants, single-cell sequencing technology has been applied to generate root cell expression maps under many experimental conditions...

Single-cell analyses and machine learning define hematopoietic progenitor and HSC-like cells derived from human PSCs.

Blood
Hematopoietic stem and progenitor cells (HSPCs) develop in distinct waves at various anatomical sites during embryonic development. The in vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates some of these processes; however, i...

Machine learning and statistical methods for clustering single-cell RNA-sequencing data.

Briefings in bioinformatics
UNLABELLED: Single-cell RNAsequencing (scRNA-seq) technologies have enabled the large-scale whole-transcriptome profiling of each individual single cell in a cell population. A core analysis of the scRNA-seq transcriptome profiles is to cluster the s...

Prediction of condition-specific regulatory genes using machine learning.

Nucleic acids research
Recent advances in genomic technologies have generated data on large-scale protein-DNA interactions and open chromatin regions for many eukaryotic species. How to identify condition-specific functions of transcription factors using these data has bec...

Uncovering the key dimensions of high-throughput biomolecular data using deep learning.

Nucleic acids research
Recent advances in high-throughput single-cell RNA-seq have enabled us to measure thousands of gene expression levels at single-cell resolution. However, the transcriptomic profiles are high-dimensional and sparse in nature. To address it, a deep lea...