AIMC Topic: Time-Lapse Imaging

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Cytoplasmic movements of the early human embryo: imaging and artificial intelligence to predict blastocyst development.

Reproductive biomedicine online
RESEARCH QUESTION: Can artificial intelligence and advanced image analysis extract and harness novel information derived from cytoplasmic movements of the early human embryo to predict development to blastocyst?

Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments.

Nature communications
Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit fluorescence microscopy's utility. Recently, it has been shown t...

CytoCensus, mapping cell identity and division in tissues and organs using machine learning.

eLife
A major challenge in cell and developmental biology is the automated identification and quantitation of cells in complex multilayered tissues. We developed CytoCensus: an easily deployed implementation of supervised machine learning that extends conv...

Discovering the hidden messages within cell trajectories using a deep learning approach for in vitro evaluation of cancer drug treatments.

Scientific reports
We describe a novel method to achieve a universal, massive, and fully automated analysis of cell motility behaviours, starting from time-lapse microscopy images. The approach was inspired by the recent successes in application of machine learning for...

Processing citizen science- and machine-annotated time-lapse imagery for biologically meaningful metrics.

Scientific data
Time-lapse cameras facilitate remote and high-resolution monitoring of wild animal and plant communities, but the image data produced require further processing to be useful. Here we publish pipelines to process raw time-lapse imagery, resulting in c...

A novel machine learning based approach for iPS progenitor cell identification.

PLoS computational biology
Identification of induced pluripotent stem (iPS) progenitor cells, the iPS forming cells in early stage of reprogramming, could provide valuable information for studying the origin and underlying mechanism of iPS cells. However, it is very difficult ...

Towards the automation of early-stage human embryo development detection.

Biomedical engineering online
BACKGROUND: Infertility and subfertility affect a significant proportion of humanity. Assisted reproductive technology has been proven capable of alleviating infertility issues. In vitro fertilisation is one such option whose success is highly depend...

Development and evaluation of inexpensive automated deep learning-based imaging systems for embryology.

Lab on a chip
Embryo assessment and selection is a critical step in an in vitro fertilization (IVF) procedure. Current embryo assessment approaches such as manual microscopy analysis done by embryologists or semi-automated time-lapse imaging systems are highly sub...

Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology.

PloS one
Growth in open-source hardware designs combined with the low-cost of high performance optoelectronic and robotics components has supported a resurgence of in-house custom lab equipment development. We describe a low cost (below $700), open-source, fu...