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Cell Lineage

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Using deep learning for quantification of cellularity and cell lineages in bone marrow biopsies and comparison to normal age-related variation.

Pathology
Cellularity estimation forms an important aspect of the visual examination of bone marrow biopsies. In clinical practice, cellularity is estimated by eye under a microscope, which is rapid, but subjective and subject to inter- and intraobserver varia...

Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.

Stem cell reports
Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive, posing ch...

Tracking cell lineages in 3D by incremental deep learning.

eLife
Deep learning is emerging as a powerful approach for bioimage analysis. Its use in cell tracking is limited by the scarcity of annotated data for the training of deep-learning models. Moreover, annotation, training, prediction, and proofreading curre...

Artificial Intelligence in Bone Marrow Histological Diagnostics: Potential Applications and Challenges.

Pathobiology : journal of immunopathology, molecular and cellular biology
The expanding digitalization of routine diagnostic histological slides holds a potential to apply artificial intelligence (AI) to pathology, including bone marrow (BM) histology. In this perspective, we describe potential tasks in diagnostics that ca...

Single-cell segmentation in bacterial biofilms with an optimized deep learning method enables tracking of cell lineages and measurements of growth rates.

Molecular microbiology
Bacteria often grow into matrix-encased three-dimensional (3D) biofilm communities, which can be imaged at cellular resolution using confocal microscopy. From these 3D images, measurements of single-cell properties with high spatiotemporal resolution...

Visualizing and Comparing Machine Learning Predictions to Improve Human-AI Teaming on the Example of Cell Lineage.

IEEE transactions on visualization and computer graphics
We visualize the predictions of multiple machine learning models to help biologists as they interactively make decisions about cell lineage-the development of a (plant) embryo from a single ovum cell. Based on a confocal microscopy dataset, tradition...

Deep learning-based models for preimplantation mouse and human embryos based on single-cell RNA sequencing.

Nature methods
The rapid growth of single-cell transcriptomic technology has produced an increasing number of datasets for both embryonic development and in vitro pluripotent stem cell-derived models. This avalanche of data surrounding pluripotency and the process ...

Deep learning-based enhancement of fluorescence labeling for accurate cell lineage tracing during embryogenesis.

Bioinformatics (Oxford, England)
MOTIVATION: Automated cell lineage tracing throughout embryogenesis plays a key role in the study of regulatory control of cell fate differentiation, morphogenesis and organogenesis in the development of animals, including nematode Caenorhabditis ele...

X-scPAE: An explainable deep learning model for embryonic lineage allocation prediction based on single-cell transcriptomics revealing key genes in embryonic cell development.

Computers in biology and medicine
In single-cell transcriptomics research, accurately predicting cell lineage allocation and identifying differences between lineages are crucial for understanding cell differentiation processes and reducing early pregnancy miscarriages in humans. This...