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Hepatocytes

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Construction and characterization of recombinant adenovirus carrying a mouse TIGIT-GFP gene.

Genetics and molecular research : GMR
Recombinant adenovirus vector systems have been used extensively in protein research and gene therapy. However, the construction and characterization of recombinant adenovirus is a tedious and time-consuming process. TIGIT is a recently discovered im...

Computational assignment of cell-cycle stage from single-cell transcriptome data.

Methods (San Diego, Calif.)
The transcriptome of single cells can reveal important information about cellular states and heterogeneity within populations of cells. Recently, single-cell RNA-sequencing has facilitated expression profiling of large numbers of single cells in para...

Quantifying co-cultured cell phenotypes in high-throughput using pixel-based classification.

Methods (San Diego, Calif.)
Biologists increasingly use co-culture systems in which two or more cell types are grown in cell culture together in order to better model cells' native microenvironments. Co-cultures are often required for cell survival or proliferation, or to maint...