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Methylation

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H2Opred: a robust and efficient hybrid deep learning model for predicting 2'-O-methylation sites in human RNA.

Briefings in bioinformatics
2'-O-methylation (2OM) is the most common post-transcriptional modification of RNA. It plays a crucial role in RNA splicing, RNA stability and innate immunity. Despite advances in high-throughput detection, the chemical stability of 2OM makes it diff...

Nm-Nano: a machine learning framework for transcriptome-wide single-molecule mapping of 2´-O-methylation (Nm) sites in nanopore direct RNA sequencing datasets.

RNA biology
2´-O-methylation (Nm) is one of the most abundant modifications found in both mRNAs and noncoding RNAs. It contributes to many biological processes, such as the normal functioning of tRNA, the protection of mRNA against degradation by the decapping a...

Interpretable Multi-Scale Deep Learning for RNA Methylation Analysis across Multiple Species.

International journal of molecular sciences
RNA modification plays a crucial role in cellular regulation. However, traditional high-throughput sequencing methods for elucidating their functional mechanisms are time-consuming and labor-intensive, despite extensive research. Moreover, existing m...

Predicting lysine methylation sites using a convolutional neural network.

Methods (San Diego, Calif.)
Protein lysine methylation is a particular type of post translational modification that plays an important role in both histone and non-histone function regulation in proteins. Deregulation caused by lysine methyltransferases has been identified as t...

BiLSTM- and CNN-Based m6A Modification Prediction Model for circRNAs.

Molecules (Basel, Switzerland)
m6A methylation, a ubiquitous modification on circRNAs, exerts a profound influence on RNA function, intracellular behavior, and diverse biological processes, including disease development. While prediction algorithms exist for mRNA m6A modifications...

Quantitative profiling N1-methyladenosine (m1A) RNA methylation from Oxford nanopore direct RNA sequencing data.

Methods (San Diego, Calif.)
With the recent advanced direct RNA sequencing technique that proposed by the Oxford Nanopore Technologies, RNA modifications can be detected and profiled in a simple and straightforward manner. Majority nanopore-based modification studies were devot...

m6ACali: machine learning-powered calibration for accurate m6A detection in MeRIP-Seq.

Nucleic acids research
We present m6ACali, a novel machine-learning framework aimed at enhancing the accuracy of N6-methyladenosine (m6A) epitranscriptome profiling by reducing the impact of non-specific antibody enrichment in MeRIP-Seq. The calibration model serves as a g...

DeepPRMS: advanced deep learning model to predict protein arginine methylation sites.

Briefings in functional genomics
Protein methylation is a form of post-translational modifications of protein, which is crucial for various cellular processes, including transcription activity and DNA repair. Correctly predicting protein methylation sites is fundamental for research...

Nmix: a hybrid deep learning model for precise prediction of 2'-O-methylation sites based on multi-feature fusion and ensemble learning.

Briefings in bioinformatics
RNA 2'-O-methylation (Nm) is a crucial post-transcriptional modification with significant biological implications. However, experimental identification of Nm sites is challenging and resource-intensive. While multiple computational tools have been de...

Integration of 101 machine learning algorithm combinations to unveil m6A/m1A/m5C/m7G-associated prognostic signature in colorectal cancer.

Scientific reports
Colorectal cancer (CRC) is the most common malignancy in the digestive system, with a lower 5-year overall survival rate. There is increasing evidence showing that RNA modification regulators such as m1A, m5C, m6A, and m7G play crucial roles in tumor...