AIMC Topic: Microscopy, Fluorescence

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Benchmarking robustness of deep neural networks in semantic segmentation of fluorescence microscopy images.

BMC bioinformatics
BACKGROUND: Fluorescence microscopy (FM) is an important and widely adopted biological imaging technique. Segmentation is often the first step in quantitative analysis of FM images. Deep neural networks (DNNs) have become the state-of-the-art tools f...

A supervised graph-based deep learning algorithm to detect and quantify clustered particles.

Nanoscale
Considerable efforts are currently being devoted to characterizing the topography of membrane-embedded proteins using combinations of biophysical and numerical analytical approaches. In this work, we present an end-to-end (, human intervention-indepe...

Structured adaptive boosting trees for detection of multicellular aggregates in fluorescence intravital microscopy.

Microvascular research
Fluorescence intravital microscopy captures large data sets of dynamic multicellular interactions within various organs such as the lungs, liver, and brain of living subjects. In medical imaging, edge detection is used to accurately identify and deli...

Breast histopathological imaging using ultra-fast fluorescence confocal microscopy to identify cancer lesions at early stage.

Microscopy research and technique
Ultrafast fluorescent confocal microscopy is a hypothetical approach for breast cancer detection because of its potential to achieve instantaneous, high-resolution images of cellular-level tissue features. Traditional approaches such as mammography a...

Nanoscale single-vesicle analysis: High-throughput approaches through AI-enhanced super-resolution image analysis.

Biosensors & bioelectronics
The analysis of membrane vesicles at the nanoscale level is crucial for advancing the understanding of intercellular communication and its implications for health and disease. Despite their significance, the nanoscale analysis of vesicles at the sing...

Clinical-Grade Validation of an Autofluorescence Virtual Staining System With Human Experts and a Deep Learning System for Prostate Cancer.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
The tissue diagnosis of adenocarcinoma and intraductal carcinoma of the prostate includes Gleason grading of tumor morphology on the hematoxylin and eosin stain and immunohistochemistry markers on the prostatic intraepithelial neoplasia-4 stain (CK5/...

Improving quantitative prediction of protein subcellular locations in fluorescence images through deep generative models.

Computers in biology and medicine
Machine learning has been employed in recognizing protein localization at the subcellular level, which highly facilitates the protein function studies, especially for those multi-label proteins that localize in more than one organelle. However, exist...

Virtual tissue microstructure reconstruction across species using generative deep learning.

PloS one
Analyzing tissue microstructure is essential for understanding complex biological systems in different species. Tissue functions largely depend on their intrinsic tissue architecture. Therefore, studying the three-dimensional (3D) microstructure of t...

Non-invasive screening of bladder cancer using digital microfluidics and FLIM technology combined with deep learning.

Journal of biophotonics
Non-invasive screening for bladder cancer is crucial for treatment and postoperative follow-up. This study combines digital microfluidics (DMF) technology with fluorescence lifetime imaging microscopy (FLIM) for urine analysis and introduces a novel ...

Deep learning-based localization algorithms on fluorescence human brain 3D reconstruction: a comparative study using stereology as a reference.

Scientific reports
3D reconstruction of human brain volumes at high resolution is now possible thanks to advancements in tissue clearing methods and fluorescence microscopy techniques. Analyzing the massive data produced with these approaches requires automatic methods...