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Microscopy

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High-Throughput, Label-Free and Slide-Free Histological Imaging by Computational Microscopy and Unsupervised Learning.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Rapid and high-resolution histological imaging with minimal tissue preparation has long been a challenging and yet captivating medical pursuit. Here, the authors propose a promising and transformative histological imaging method, termed computational...

Deep learning is widely applicable to phenotyping embryonic development and disease.

Development (Cambridge, England)
Genome editing simplifies the generation of new animal models for congenital disorders. However, the detailed and unbiased phenotypic assessment of altered embryonic development remains a challenge. Here, we explore how deep learning (U-Net) can auto...

Evaluation of an Artificial Intelligence-Augmented Digital System for Histologic Classification of Colorectal Polyps.

JAMA network open
IMPORTANCE: Colorectal polyps are common, and their histopathologic classification is used in the planning of follow-up surveillance. Substantial variation has been observed in pathologists' classification of colorectal polyps, and improved assessmen...

A deep learning-based segmentation pipeline for profiling cellular morphodynamics using multiple types of live cell microscopy.

Cell reports methods
MOTIVATION: Quantitative studies of cellular morphodynamics rely on extracting leading-edge velocity time series based on accurate cell segmentation from live cell imaging. However, live cell imaging has numerous challenging issues regarding accurate...

Practical machine learning for disease diagnosis.

Cell reports methods
Deep learning neural networks are a powerful tool in the analytical toolbox of modern microscopy, but they come with an exacting requirement for accurately annotated, ground truth cell images. Otesteanu et al. (2021) elegantly streamline this process...

Deep learning for bone marrow cell detection and classification on whole-slide images.

Medical image analysis
Bone marrow (BM) examination is an essential step in both diagnosing and managing numerous hematologic disorders. BM nucleated differential count (NDC) analysis, as part of BM examination, holds the most fundamental and crucial information. However, ...

Effectiveness of Create ML in microscopy image classifications: a simple and inexpensive deep learning pipeline for non-data scientists.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Observing chromosomes is a time-consuming and labor-intensive process, and chromosomes have been analyzed manually for many years. In the last decade, automated acquisition systems for microscopic images have advanced dramatically due to advances in ...

Yeast cell segmentation in microstructured environments with deep learning.

Bio Systems
Cell segmentation is a major bottleneck in extracting quantitative single-cell information from microscopy data. The challenge is exasperated in the setting of microstructured environments. While deep learning approaches have proven useful for genera...

A review for cell and particle tracking on microscopy images using algorithms and deep learning technologies.

Biomedical journal
Time-lapse microscopy images generated by biological experiments have been widely used for observing target activities, such as the motion trajectories and survival states. Based on these observations, biologists can conclude experimental results or ...

Quantitative neuronal morphometry by supervised and unsupervised learning.

STAR protocols
We present a protocol to characterize the morphological properties of individual neurons reconstructed from microscopic imaging. We first describe a simple procedure to extract relevant morphological features from digital tracings of neural arbors. T...