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RNA, Messenger

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SiRNA silencing efficacy prediction based on a deep architecture.

BMC genomics
BACKGROUND: Small interfering RNA (siRNA) can be used to post-transcriptional gene regulation by knocking down targeted genes. In functional genomics, biomedical research and cancer therapeutics, siRNA design is a critical research topic. Various com...

Machine learning approaches infer vitamin D signaling: Critical impact of vitamin D receptor binding within topologically associated domains.

The Journal of steroid biochemistry and molecular biology
The vitamin D-modulated transcriptome of highly responsive human cells, such as THP-1 monocytes, comprises more than 500 genes, half of which are primary targets. Recently, we proposed a chromatin model of vitamin D signaling demonstrating that nearl...

A Supervised Ensemble Approach for Sensitive microRNA Target Prediction.

IEEE/ACM transactions on computational biology and bioinformatics
MicroRNAs, a class of small non-coding RNAs, regulate important biological functions via post-transcriptional regulation of messenger RNAs (mRNAs). Despite rapid development in miRNA research, precise experimental methods to determine miRNA target in...

miRAW: A deep learning-based approach to predict microRNA targets by analyzing whole microRNA transcripts.

PLoS computational biology
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by binding to partially complementary regions within the 3'UTR of their target genes. Computational methods play an important role in target prediction and assume that the miR...

Tiresias: Context-sensitive Approach to Decipher the Presence and Strength of MicroRNA Regulatory Interactions.

Theranostics
MicroRNAs (miRNAs) are short non-coding RNAs that regulate expression of target messenger RNAs (mRNAs) post-transcriptionally. Understanding the precise regulatory role of miRNAs is of great interest since miRNAs have been shown to play an important ...

Effect of bovine lactoferrin and human lactoferrin on the proliferative activity of the osteoblast cell line MC3T3-E1 in vitro.

Journal of dairy science
We conducted a comparative in vitro study on the proliferative effects of natural human lactoferrin (nhLF) and bovine lactoferrin (bLF) on osteoblasts. We investigated cell proliferation, cell survival, cell cycle, and mRNA and protein expression of ...

Monitoring bottlenose dolphin leukocyte cytokine mRNA responsiveness by qPCR.

PloS one
Both veterinarians caring for dolphins in managed populations and researchers monitoring wild populations use blood-based diagnostics to monitor bottlenose dolphin (Tursiops truncatus) health. Quantitative PCR (qPCR) can be used to assess cytokine tr...

Unsupervised Network Analysis of the Plastic Supraoptic Nucleus Transcriptome Predicts Caprin2 Regulatory Interactions.

eNeuro
The supraoptic nucleus (SON) is a group of neurons in the hypothalamus responsible for the synthesis and secretion of the peptide hormones vasopressin and oxytocin. Following physiological cues, such as dehydration, salt-loading and lactation, the SO...