AIMC Topic: Sequence Analysis, RNA

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ModiDeC: a multi-RNA modification classifier for direct nanopore sequencing.

Nucleic acids research
RNA modifications play a crucial role in various cellular functions. Here, we present ModiDeC, a deep-learning-based classifier able to identify and distinguish multiple RNA modifications (N6-methyladenosine, inosine, pseudouridine, 2'-O-methylguanos...

Differentiable graph clustering with structural grouping for single-cell RNA-seq data.

Bioinformatics (Oxford, England)
MOTIVATION: Clustering cells into subpopulations is one of the most crucial tasks in single-cell RNA sequencing (scRNA-seq) data analysis, which provides support for biological research at cellular level. With the development of graph neural networks...

Artificial intelligence approaches for tumor phenotype stratification from single-cell transcriptomic data.

eLife
Single-cell RNA-sequencing (scRNA-seq) coupled with robust computational analysis facilitates the characterization of phenotypic heterogeneity within tumors. Current scRNA-seq analysis pipelines are capable of identifying a myriad of malignant and no...

GeneDX-PBMC: An adversarial autoencoder framework for unlocking Alzheimer's disease biomarkers using blood single-cell RNA sequencing data.

Computers in biology and medicine
OBJECTIVE: To identify blood-based biomarkers and therapeutic targets for Alzheimer's disease (AD) by leveraging single-cell RNA sequencing (scRNA-seq) data from peripheral blood mononuclear cells (PBMCs) and advanced deep learning techniques.

CorrAdjust unveils biologically relevant transcriptomic correlations by efficiently eliminating hidden confounders.

Nucleic acids research
Correcting for confounding variables is often overlooked when computing RNA-RNA correlations, even though it can profoundly affect results. We introduce CorrAdjust, a method for identifying and correcting such hidden confounders. CorrAdjust selects a...

Machine learning using scRNA-seq Combined with bulk-seq to identify lactylation-related hub genes in carotid arteriosclerosis.

Scientific reports
Atherosclerosis is a chronic inflammatory disease, this study aims to investigate the immune landscape in carotid atherosclerotic plaque formation and explore diagnostic biomarkers of lactylation-associated genes, so as to gain new insights into unde...

Scmaskgan: masked multi-scale CNN and attention-enhanced GAN for scRNA-seq dropout imputation.

BMC bioinformatics
Single-cell RNA sequencing (scRNA-seq) enables high-resolution analysis of cellular heterogeneity, but dropout events, where gene expression is undetected in individual cells, present a significant challenge. We propose scMASKGAN, which transforms ma...

Single-cell RNA sequencing reveals immunological link between house dust mite allergy and childhood asthma.

Scientific reports
Allergic asthma in children is typically associated with house dust mites (HDM) as the key allergen. Nevertheless, the diagnostic rate remains below 60% due to the absence of specific symptoms and diagnostic markers, which hinders the implementation ...

Peak analysis of cell-free RNA finds recurrently protected narrow regions with clinical potential.

Genome biology
BACKGROUND: Cell-free RNAs (cfRNAs) can be detected in biofluids and have emerged as valuable disease biomarkers. Accurate identification of the fragmented cfRNA signals, especially those originating from pathological cells, is crucial for understand...

Nonparametric IPSS: fast, flexible feature selection with false discovery control.

Bioinformatics (Oxford, England)
MOTIVATION: Feature selection is a critical task in machine learning and statistics. However, existing feature selection methods either (i) rely on parametric methods such as linear or generalized linear models, (ii) lack theoretical false discovery ...