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Bacteria

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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms.

Frontiers in cellular and infection microbiology
INTRODUCTION: Accurately assessing biofilm viability is essential for evaluating both biofilm formation and the efficacy of antibacterial treatments. Traditional SYTO9 and propidium iodide (PI) live/dead staining in biofilm viability assays often ace...

Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study.

Frontiers in cellular and infection microbiology
BACKGROUND: Though droplet digital PCR (ddPCR) has emerged as a promising tool for early pathogen detection in bloodstream infections (BSIs), more studies are needed to support its clinical application widely due to different ddPCR platforms with dis...

Gut microbial composition associated with risk of premature aging in women with Yin-deficiency constitution.

Frontiers in cellular and infection microbiology
BACKGROUND: Yin-deficiency constitution (YinDC) refers to a traditional Chinese medicine concept, characterized by an imbalance state that includes an imbalance in the gut microbiota, resulting from a relative deficiency of Yin fluids within the body...

Interpretation of Bacterial Smears and Cultures Using Artificial Intelligence.

Clinics in laboratory medicine
The use of artificial intelligence (AI) to aid in the diagnosis of infectious diseases is a growing area of interest. AI-based applications in the clinical microbiology laboratory have shown great prospect for the automated interpretation of smears a...

Predicting biomass conversion and COD removal in wastewater treatment by phototrophic bacteria with interpretable machine learning.

Journal of environmental management
Photosynthetic bacteria (PSB) excel in wastewater treatment by removing pollutants and generating biomass but are challenging to optimize due to complex operational and environmental interactions. Neural Ordinary Differential Equations, Elastic Net, ...

Robotic versus manual disinfection of global priority pathogens at COVID-19-dedicated hospitals.

American journal of infection control
BACKGROUND: Twelve bacterial families identified as global priority pathogens (GPPs) pose the greatest threat to human health due to declining antibiotic efficacy. Robotics, a swift and contactless tool for disinfecting hospital surfaces, was sought ...

Automatic visual detection of activated sludge microorganisms based on microscopic phase contrast image optimisation and deep learning.

Journal of microscopy
The types and quantities of microorganisms in activated sludge are directly related to the stability and efficiency of sewage treatment systems. This paper proposes a sludge microorganism detection method based on microscopic phase contrast image opt...

Bacteria Flagella-Mimicking Polymer Multilayer Magnetic Microrobots.

Small methods
Mass production of biomedical microrobots demands expensive and complex preparation techniques and versatile biocompatible materials. Learning from natural bacteria flagella, the study demonstrates a magnetic polymer multilayer cylindrical microrobot...

Explainable deep learning and virtual evolution identifies antimicrobial peptides with activity against multidrug-resistant human pathogens.

Nature microbiology
Artificial intelligence (AI) is a promising approach to identify new antimicrobial compounds in diverse microbial species. Here we developed an AI-based, explainable deep learning model, EvoGradient, that predicts the potency of antimicrobial peptide...

Reviewing on AI-Designed Antibiotic Targeting Drug-Resistant Superbugs by Emphasizing Mechanisms of Action.

Drug development research
The emergence of drug-resistant bacteria, often referred to as "superbugs," poses a profound and escalating challenge to global health systems, surpassing the capabilities of traditional antibiotic discovery methods. As resistance mechanisms evolve r...