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Bacterial Proteins

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Development and Application of Two Inducible Expression Systems for Streptococcus suis.

Microbiology spectrum
Streptococcus suis is an important zoonotic bacterial pathogen posing a threat to the pig industry as well as public health, for which the mechanisms of growth and cell division remain largely unknown. Developing convenient genetic tools that can ach...

Soft Sensor Modeling Method Based on Improved KH-RBF Neural Network Bacteria Concentration in Marine Alkaline Protease Fermentation Process.

Applied biochemistry and biotechnology
Marine alkaline protease (MAP) fermentation is a complex multivariable, multi-coupled, and nonlinear process. Some unmeasured parameters will affect the quality of protease. Aiming at the problem that some parameters are difficult to be detected onli...

Predicting the future of excitation energy transfer in light-harvesting complex with artificial intelligence-based quantum dynamics.

Nature communications
Exploring excitation energy transfer (EET) in light-harvesting complexes (LHCs) is essential for understanding the natural processes and design of highly-efficient photovoltaic devices. LHCs are open systems, where quantum effects may play a crucial ...

NIFtHool: an informatics program for identification of NifH proteins using deep neural networks.

F1000Research
Atmospheric nitrogen fixation carried out by microorganisms has environmental and industrial importance, related to the increase of soil fertility and productivity. The present work proposes the development of a new high precision system that allows ...

Few-fs resolution of a photoactive protein traversing a conical intersection.

Nature
The structural dynamics of a molecule are determined by the underlying potential energy landscape. Conical intersections are funnels connecting otherwise separate potential energy surfaces. Posited almost a century ago, conical intersections remain t...

TwinCons: Conservation score for uncovering deep sequence similarity and divergence.

PLoS computational biology
We have developed the program TwinCons, to detect noisy signals of deep ancestry of proteins or nucleic acids. As input, the program uses a composite alignment containing pre-defined groups, and mathematically determines a 'cost' of transforming one ...

Deep protein representations enable recombinant protein expression prediction.

Computational biology and chemistry
A crucial process in the production of industrial enzymes is recombinant gene expression, which aims to induce enzyme overexpression of the genes in a host microbe. Current approaches for securing overexpression rely on molecular tools such as adjust...

Application of the random forest algorithm to Streptococcus pyogenes response regulator allele variation: from machine learning to evolutionary models.

Scientific reports
Group A Streptococcus (GAS) is a globally significant bacterial pathogen. The GAS genotyping gold standard characterises the nucleotide variation of emm, which encodes a surface-exposed protein that is recombinogenic and under immune-based selection ...

Evaluation of the VITEK 2 Advanced Expert System performance for predicting resistance mechanisms in Enterobacterales acquired from a hospital-based screening program.

Pathology
There is limited literature examining the accuracy of the VITEK 2 Advanced Expert System (AES) in characterisation of β-lactamase resistance patterns. We present a prospective single centre study to better ascertain the performance characteristics of...

Antibiofilm Activity of α-Amylase from Bacillus subtilis and Prediction of the Optimized Conditions for Biofilm Removal by Response Surface Methodology (RSM) and Artificial Neural Network (ANN).

Applied biochemistry and biotechnology
α-amylase is known to have antibiofilm activity against biofilms of both Gram positive and Gram-negative bacterial strains. Partially purified α-amylase from Bacillus subtilis was found to have inhibit biofilm formed by P. aeruginosa and S. aureus. T...