AIMC Topic: CRISPR-Cas Systems

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Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing.

ACS nano Dec 2, 2024
The development of novel detection technology for meat species authenticity is imperative. Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet platform for meat species authenticity detection named CC-drop (RISPR/C...
Animals Biosensing Techniques Food Analysis Machine Learning Endodeoxyribonucleases CRISPR-Associated Proteins Nucleic Acids Meat CRISPR-Cas Systems Bacterial Proteins
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Deciphering Cas9 specificity: Role of domain dynamics and RNA:DNA hybrid interactions revealed through machine learning and accelerated molecular simulations.

International journal of biological macromolecules Nov 19, 2024
CRISPR/Cas9 technology is widely used for gene editing, but off-targeting still remains a major concern in therapeutic applications. Although Cas9 variants with better mismatch discrimination have been developed, they have significantly lower rates o...
CRISPR-Cas Systems Bacterial Proteins Protein Domains Machine Learning DNA Substrate Specificity CRISPR-Associated Protein 9 Francisella Molecular Dynamics Simulation RNA
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Balanced Training Sets Improve Deep Learning-Based Prediction of CRISPR sgRNA Activity.

ACS synthetic biology Nov 4, 2024
CRISPR-Cas systems have transformed the field of synthetic biology by providing a versatile method for genome editing. The efficiency of CRISPR systems is largely dependent on the sequence of the constituent sgRNA, necessitating the development of co...
RNA, Guide, CRISPR-Cas Systems Neural Networks, Computer Deep Learning CRISPR-Cas Systems Yarrowia Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
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Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs.

Nature methods Sep 23, 2024
Transposon (IS200/IS605)-encoded TnpB proteins are predecessors of class 2 type V CRISPR effectors and have emerged as one of the most compact genome editors identified thus far. Here, we optimized the design of Deinococcus radiodurans (ISDra2) TnpB ...
HEK293 Cells Gene Editing DNA Transposable Elements RNA, Guide, CRISPR-Cas Systems Deinococcus Deep Learning Humans Mice Animals CRISPR-Cas Systems
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The transformative potential of AI-driven CRISPR-Cas9 genome editing to enhance CAR T-cell therapy.

Computers in biology and medicine Sep 10, 2024
This narrative review examines the promising potential of integrating artificial intelligence (AI) with CRISPR-Cas9 genome editing to advance CAR T-cell therapy. AI algorithms offer unparalleled precision in identifying genetic targets, essential for...
Neoplasms CRISPR-Cas Systems Gene Editing Artificial Intelligence Receptors, Chimeric Antigen Immunotherapy, Adoptive Humans
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High-Throughput and Integrated CRISPR/Cas12a-Based Molecular Diagnosis Using a Deep Learning Enabled Microfluidic System.

ACS nano Aug 22, 2024
CRISPR/Cas-based molecular diagnosis demonstrates potent potential for sensitive and rapid pathogen detection, notably in SARS-CoV-2 diagnosis and mutation tracking. Yet, a major hurdle hindering widespread practical use is its restricted throughput,...
Nucleic Acid Amplification Techniques CRISPR-Cas Systems Mutation Lab-On-A-Chip Devices Microfluidic Analytical Techniques SARS-CoV-2 Deep Learning Humans Molecular Diagnostic Techniques COVID-19
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CRISPR-Enhanced Photocurrent Polarity Switching for Dual-lncRNA Detection Combining Deep Learning for Cancer Diagnosis.

Analytical chemistry Aug 2, 2024
Abnormal expression in long noncoding RNAs (lncRNAs) is closely associated with cancers. Herein, a novel CRISPR/Cas13a-enhanced photocurrent-polarity-switching photoelectrochemical (PEC) biosensor was engineered for the joint detection of dual lncRNA...
Humans Electrochemical Techniques Limit of Detection CRISPR-Cas Systems Cadmium Compounds Sulfides Photochemical Processes Biosensing Techniques RNA, Long Noncoding Quantum Dots Neoplasms Deep Learning
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Rapid, portable, and sensitive detection of CaMV35S by RPA-CRISPR/Cas12a-G4 colorimetric assays with high accuracy deep learning object recognition and classification.

Talanta Jun 20, 2024
Fast, sensitive, and portable detection of genetic modification contributes to agricultural security and food safety. Here, we developed RPA-CRISPR/Cas12a-G-quadruplex colorimetric assays that can combine with intelligent recognition by deep learning...
Bacterial Proteins Endodeoxyribonucleases CRISPR-Associated Proteins G-Quadruplexes Deep Learning Limit of Detection Colorimetry Promoter Regions, Genetic CRISPR-Cas Systems
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Identification of Family-Specific Features in Cas9 and Cas12 Proteins: A Machine Learning Approach Using Complete Protein Feature Spectrum.

Journal of chemical information and modeling Jun 5, 2024
The recent development of CRISPR-Cas technology holds promise to correct gene-level defects for genetic diseases. The key element of the CRISPR-Cas system is the Cas protein, a nuclease that can edit the gene of interest assisted by guide RNA. Howeve...
CRISPR-Cas Systems CRISPR-Associated Proteins CRISPR-Associated Protein 9 Machine Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning.

Journal of visualized experiments : JoVE May 10, 2024
We report a fast, easy-to-implement, highly sensitive, sequence-specific, and point-of-care (POC) DNA virus detection system, which combines recombinase polymerase amplification (RPA) and CRISPR/Cas12a system for trace detection of DNA viruses. Targe...
Recombinases DNA, Viral DNA Viruses Ranavirus Point-of-Care Systems Nucleic Acid Amplification Techniques Deep Learning CRISPR-Cas Systems
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