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Culture Media

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Optimizing the production and efficacy of antimicrobial bioactive compounds from in combating multi-drug-resistant pathogens.

Frontiers in cellular and infection microbiology
BACKGROUND: The rise of antibiotic-resistant pathogens has intensified the search for novel antimicrobial agents. This study aimed to isolate from local soil samples and evaluate its antimicrobial properties, along with optimizing the production of ...

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle.

PloS one
A successful regeneration protocol was developed for micropropagation of Lilium akkusianum R. Gämperle, an endemic species of Türkiye, from scale explants. The study also aimed to evaluate the effects of Meta-Topolin (mT) and N6-Benzyladenine (BA) on...

Development and evaluation of an artificial intelligence for bacterial growth monitoring in clinical bacteriology.

Journal of clinical microbiology
In clinical bacteriology laboratories, reading and processing of sterile plates remain a significant part of the routine workload (30%-40% of the plates). Here, an algorithm was developed for bacterial growth detection starting with any type of speci...

Contribution of machine learning for subspecies identification from Mycobacterium abscessus with MALDI-TOF MS in solid and liquid media.

Microbial biotechnology
Mycobacterium abscessus (MABS) displays differential subspecies susceptibility to macrolides. Thus, identifying MABS's subspecies (M. abscessus, M. bolletii and M. massiliense) is a clinical necessity for guiding treatment decisions. We aimed to asse...

Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches.

Biotechnology advances
The production of monoclonal antibodies (mAbs) using Chinese Hamster Ovary (CHO) cells has revolutionized the treatment of numerous diseases, solidifying their position as a cornerstone of the biopharmaceutical industry. However, achieving maximum mA...

Extreme learning machine for identifying soil-dwelling microorganisms cultivated on agar media.

Scientific reports
The aim of this research is to create an automated system for identifying soil microorganisms at the genera level based on raw microscopic images of monocultural colonies grown in laboratory environment. The examined genera are: Fusarium, Trichoderma...

Data-driven discovery of the interplay between genetic and environmental factors in bacterial growth.

Communications biology
A complex interplay of genetic and environmental factors influences bacterial growth. Understanding these interactions is crucial for insights into complex living systems. This study employs a data-driven approach to uncover the principles governing ...

Rapid and high accuracy identification of culture medium by CNN of Raman spectra.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Culture media are widely used for biological research and production. It is essential for the growth of microorganisms, cells, or tissues. It includes complex components like carbohydrates, proteins, vitamins, and minerals. The media's consistency is...

Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida.

Communications biology
Although synthetic biology can produce valuable chemicals in a renewable manner, its progress is still hindered by a lack of predictive capabilities. Media optimization is a critical, and often overlooked, process which is essential to obtain the tit...

Deep Learning-Powered Colloidal Digital SERS for Precise Monitoring of Cell Culture Media.

Nano letters
Maintaining consistent quality in biomanufacturing is essential for producing high-quality complex biologics. Yet, current process analytical technologies (PAT) often fall short in achieving rapid and accurate monitoring of small-molecule critical pr...