AIMC Topic: Flow Cytometry

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Cold storage surpasses the impact of biological age and donor characteristics on red blood cell morphology classified by deep machine learning.

Scientific reports
Assessment of the morphology of red blood cells (RBCs) can improve clinical benefits following blood transfusion. Deep machine learning surpasses traditional microscopy-based classification methods, offering more accurate and consistent results while...

Predicting cell properties with AI from 3D imaging flow cytometer data.

Scientific reports
Predicting the properties of tissues or organisms from the genomics data is widely accepted by the medical community. Here we ask a question: can we predict the properties of each individual cell? Single-cell genomics does not work because the RNA se...

Neuromorphic-enabled video-activated cell sorting.

Nature communications
Imaging flow cytometry allows image-activated cell sorting (IACS) with enhanced feature dimensions in cellular morphology, structure, and composition. However, existing IACS frameworks suffer from the challenges of 3D information loss and processing ...

An integrated microflow cytometry platform with artificial intelligence capabilities for point-of-care cellular phenotype analysis.

Biosensors & bioelectronics
The EZ DEVICE is an integrated fluorescence microflow cytometer designed for automated cell phenotyping and enumeration using artificial intelligence (AI). The platform consists of a laser diode, optical filter, objective lens, CMOS image sensor, and...

Machine learning based on multiplatform tests assists in subtype classification of mature B-cell neoplasms.

British journal of haematology
Mature B-cell neoplasms (MBNs) are clonal proliferative diseases encompassing over 40 subtypes. The WHO classification (morphology, immunology, cytogenetics and molecular biology) provides comprehensive diagnostic understandings. However, MBN subtypi...

Deep Learning-Assisted Label-Free Parallel Cell Sorting with Digital Microfluidics.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Sorting specific cells from heterogeneous samples is important for research and clinical applications. In this work, a novel label-free cell sorting method is presented that integrates deep learning image recognition with microfluidic manipulation to...

Neural Network-Enabled Multiparametric Impedance Signal Templating for High throughput Single-Cell Deformability Cytometry Under Viscoelastic Extensional Flows.

Small (Weinheim an der Bergstrasse, Germany)
Cellular biophysical metrics exhibit systematic alterations during processes, such as metastasis and immune cell activation, which can be used to identify and separate live cell subpopulations for targeting drug screening. Image-based biophysical cyt...

Artificial intelligence in Andrological flow cytometry: The next step?

Animal reproduction science
Since its introduction in animal andrology, flow cytometry (FC) has dramatically evolved. Nowadays, many compartments and functions of the spermatozoa can be analyzed in thousands of spermatozoa, including, but not limited to DNA, acrosome, membrane ...

Parameter optimization for stable clustering using FlowSOM: a case study from CyTOF.

Frontiers in immunology
High-dimensional cell phenotyping is a powerful tool to study molecular and cellular changes in health and diseases. CyTOF enables high-dimensional cell phenotyping using tens of surface and intra-cellular markers. To utilize the full potential of Cy...

An AI-based imaging flow cytometry approach to study erythrophagocytosis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Erythrophagocytosis is a process consisting of recognition, engulfment and digestion by phagocytes of antibody-coated or damaged erythrocytes. Understanding the dynamics that are behind erythrophagocytosis is fundamental to comprehend this cellular p...