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Golgi Apparatus

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Digging deep into Golgi phenotypic diversity with unsupervised machine learning.

Molecular biology of the cell
The synthesis of glycans and the sorting of proteins are critical functions of the Golgi apparatus and depend on its highly complex and compartmentalized architecture. High-content image analysis coupled to RNA interference screening offers opportuni...

isGPT: An optimized model to identify sub-Golgi protein types using SVM and Random Forest based feature selection.

Artificial intelligence in medicine
The Golgi Apparatus (GA) is a key organelle for protein synthesis within the eukaryotic cell. The main task of GA is to modify and sort proteins for transport throughout the cell. Proteins permeate through the GA on the ER (Endoplasmic Reticulum) fac...

Robust classification of cell cycle phase and biological feature extraction by image-based deep learning.

Molecular biology of the cell
Across the cell cycle, the subcellular organization undergoes major spatiotemporal changes that could in principle contain biological features that could potentially represent cell cycle phase. We applied convolutional neural network-based classifier...

Machine-learning assisted confocal imaging of intracellular sites of triglycerides and cholesteryl esters formation and storage.

Analytica chimica acta
All living systems are maintained by a constant flux of metabolic energy and, among the different reactions, the process of lipids storage and lipolysis is of fundamental importance. Current research has focused on the investigation of lipid droplets...

Deep neural network automated segmentation of cellular structures in volume electron microscopy.

The Journal of cell biology
Volume electron microscopy is an important imaging modality in contemporary cell biology. Identification of intracellular structures is a laborious process limiting the effective use of this potentially powerful tool. We resolved this bottleneck with...

A Primer on Deep Learning-Based Cellular Image Classification of Changes in the Spatial Distribution of the Golgi Apparatus After Experimental Manipulation.

Methods in molecular biology (Clifton, N.J.)
The visual classification of cell images according to differences in the spatial patterns of subcellular structure is an important methodology in cell and developmental biology. Experimental perturbation of cell function can induce changes in the spa...

A CNN-GNN Approach for Polarity Vectors Prediction in 3D Microscopy Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
The polarity between nuclei and Golgi is an important aspect of cellular division, migration and signaling. For example, nucleus-Golgi polarity significantly impacts angiogenesis, the physiological process in which new blood vessels develop from pre-...