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Cell Polarity

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Mapping Cellular Polarity Networks Using Mass Spectrometry-based Strategies.

Journal of molecular biology
Cell polarity is a vital biological process involved in the building, maintenance and normal functioning of tissues in invertebrates and vertebrates. Unsurprisingly, molecular defects affecting polarity organization and functions have a strong impact...

Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro.

Stem cell reports
Stem cell-based embryo models by cultured pluripotent and extra-embryonic lineage stem cells are novel platforms to model early postimplantation development. We showed that induced pluripotent stem cells (iPSCs) could form ITS (iPSCs and trophectoder...

Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space.

PLoS computational biology
Navigation of fast migrating cells such as amoeba Dictyostelium and immune cells are tightly associated with their morphologies that range from steady polarized forms that support high directionality to those more complex and variable when making fre...

Spatial and temporal dynamics of RhoA activities of single breast tumor cells in a 3D environment revealed by a machine learning-assisted FRET technique.

Experimental cell research
One of the hallmarks of cancer cells is their exceptional ability to migrate within the extracellular matrix (ECM) for gaining access to the circulatory system, a critical step of cancer metastasis. RhoA, a small GTPase, is known to be a key molecula...

Stain-free detection of embryo polarization using deep learning.

Scientific reports
Polarization of the mammalian embryo at the right developmental time is critical for its development to term and would be valuable in assessing the potential of human embryos. However, tracking polarization requires invasive fluorescence staining, im...

Analysis of cortical cell polarity by imaging flow cytometry.

Journal of cellular biochemistry
Metastasis is the main cause of cancer-related death and therapies specifically targeting metastasis are highly needed. Cortical cell polarity (CCP) is a prometastatic property of circulating tumor cells affecting their ability to exit blood vessels ...

A CNN-GNN Approach for Polarity Vectors Prediction in 3D Microscopy Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
The polarity between nuclei and Golgi is an important aspect of cellular division, migration and signaling. For example, nucleus-Golgi polarity significantly impacts angiogenesis, the physiological process in which new blood vessels develop from pre-...