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Real-Time Polymerase Chain Reaction

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Comparison of Xpert MTB/RIF (G4) and Xpert Ultra, including trace readouts, for the diagnosis of pulmonary tuberculosis in a TB and HIV endemic setting.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
BACKGROUND: There are limited data about Xpert-Ultra performance in different settings, in HIV-infected persons, in those with a history of previous TB, and with trace readouts.

Detection of COVID-19 Infection from Routine Blood Exams with Machine Learning: A Feasibility Study.

Journal of medical systems
The COVID-19 pandemia due to the SARS-CoV-2 coronavirus, in its first 4 months since its outbreak, has to date reached more than 200 countries worldwide with more than 2 million confirmed cases (probably a much higher number of infected), and almost ...

Amplification Curve Analysis: Data-Driven Multiplexing Using Real-Time Digital PCR.

Analytical chemistry
Information about the kinetics of PCR reactions is encoded in the amplification curve. However, in digital PCR (dPCR), this information is typically neglected by collapsing each amplification curve into a binary output (positive/negative). Here, we d...

Anomaly Identification during Polymerase Chain Reaction for Detecting SARS-CoV-2 Using Artificial Intelligence Trained from Simulated Data.

Molecules (Basel, Switzerland)
Real-time reverse transcription (RT) PCR is the gold standard for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), owing to its sensitivity and specificity, thereby meeting the demand for the rising number of cases. The scarcit...

Machine Learning Analysis of MicroRNA Expression Data Reveals Novel Diagnostic Biomarker for Ischemic Stroke.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association
OBJECTIVES: Ischemic stroke (IS) is one of the leading causes of morbidity and mortality worldwide. Circulating microRNAs have a potential as minimally invasive biomarkers for disease prediction, diagnosis, and prognosis. In this study, we sought to ...

Specificity of SARS-CoV-2 Real-Time PCR Improved by Deep Learning Analysis.

Journal of clinical microbiology
Real-time PCR (RT-PCR) is widely used to diagnose human pathogens. RT-PCR data are traditionally analyzed by estimating the threshold cycle ( ) at which the fluorescence signal produced by emission of a probe crosses a baseline level. Current models ...

A new dynamic deep learning noise elimination method for chip-based real-time PCR.

Analytical and bioanalytical chemistry
Point-of-care (POC) real-time polymerase chain reaction (PCR) has become one of the most important technologies for many fields such as pathogen detection and water-quality monitoring. POC real-time PCR usually adopts chips with small-volume chambers...

An automated, fully-integrated nucleic acid analyzer based on microfluidic liquid handling robot technique.

Analytica chimica acta
On-site nucleic acid testing (NAT) plays an important role for disease monitoring and pathogen diagnosis. In this work, we developed an automated and fully-integrated nucleic acid analyzer by combining the automated liquid handling robot technique wi...