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Recombinant Proteins

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[Process Optimization of PEGylating Fused Protein of LL-37 and Interferon-α2a].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
PEGylating is an effective way for prolonging the half-time period and decreasing the immunogenicity of protein drugs. With experiments of single factor, it was proved that the optimal processes for PEGylating the fused protein of LL-37 and interfero...

[Cloning, expression, and purification of c-di-AMP metabolism-related genes from Porphyromonas gingivalis].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
OBJECTIVE: To clone, express, and purify cyclic diadenosine monophosphate (c-di-AMP) metabolism-related genes from Porphyromonas gingivalis (P. gingivalis) ATCC33277.

[Effects of exogenous recombinant human basic fibroblast growth factor on the healing of muscles in rats after deep tissue injury of pressure ulcers].

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
OBJECTIVE: To investigate the effect of exogenous recombinant human basic fibroblast growth factor (rhbFGF) on the healing of muscles in rats after deep tissue injury of pressure ulcers.

Efficacy and Safety Evaluation on Arterial Thrombolysis in Treating Acute Cerebral Infarction.

Cell biochemistry and biophysics
The objective of this study was to evaluate the efficacy and safety of intra-arterial thrombolysis in treating acute cerebral infarction and further discuss the indications of acute cerebral infarction treatment, in order to enhance the therapeutic e...

[Signifiance of brush sign on susceptibility-weighted imaging predicts hemorrhagic transformation after intravenous thrombolysis in patients with acute ischemic stroke].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
OBJECTIVE: To assess brush sign (BS) on susceptibility-weighted imaging (SWI) in prediction of hemorrhagic transformation (HT) in patients with acute ischemic stroke (AIS) after intravenous thrombolysis(IVT).

Prediction of recombinant protein overexpression in Escherichia coli using a machine learning based model (RPOLP).

Computers in biology and medicine
Recombinant protein overexpression, an important biotechnological process, is ruled by complex biological rules which are mostly unknown, is in need of an intelligent algorithm so as to avoid resource-intensive lab-based trial and error experiments i...

The potential of random forest and neural networks for biomass and recombinant protein modeling in Escherichia coli fed-batch fermentations.

Biotechnology journal
Product quality assurance strategies in production of biopharmaceuticals currently undergo a transformation from empirical "quality by testing" to rational, knowledge-based "quality by design" approaches. The major challenges in this context are the ...

Neural network models - a novel tool for predicting the efficacy of growth hormone (GH) therapy in children with short stature.

Neuro endocrinology letters
INTRODUCTION: The leading method for prediction of growth hormone (GH) therapy effectiveness are multiple linear regression (MLR) models. Best of our knowledge, we are the first to apply artificial neural networks (ANN) to solve this problem. For ANN...

Robotic high-throughput purification of affinity-tagged recombinant proteins.

Methods in molecular biology (Clifton, N.J.)
Affinity purification of recombinant proteins has become the method of choice to obtain good quantities and qualities of proteins for a variety of downstream biochemical applications. While manual or FPLC-assisted purification techniques are generall...

[Retreatment strategies for the patients with HCV infection].

Przeglad epidemiologiczny
Despite recent advances in the treatment of chronic hepatitis C, especially introduction of combined therapy with pegylated interferons with ribavirin, significant number of patients fails to achieve sustained virologic response. Such patients may be...