AIMC Topic: Recombinant Proteins

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[rhPDGF-BB promotes migration of bone marrow stromal stem cells in diabetic rats].

Shanghai kou qiang yi xue = Shanghai journal of stomatology
PURPOSE: To evaluate the effect of different concentrations of rhPDGF-BB on bone marrow stromal stem cells(BMSCs) migration in diabetic rat, and related mechanisms of SDF-1 and its G-protein conjugated receptor regulation, provide a theoretical basis...

[Single-chain antibodies from alpaca for the detection of Fasciola hepatica antigens].

Revista peruana de medicina experimental y salud publica
OBJECTIVES.: To produce recombinant single-chain antibodies from alpaca that will bind to the excreted-secreted (ES) Fasciola hepatica antigen with high affinity and specificity, so as to develop new diagnostic technologies of human and animal fascio...

[Construction and high-density fermentation of alkaline pectate lyase high-yield yeast].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Pectate lyase is widely applied in ramie degumming and fabric bioscouring in the textile industry. Compared to conventional processes that involve high alkaline and high temperature treatment, enzyme based treatments have significant advantages in fi...

Prokaryotic Expression of Hepatitis C Virus-NS3 Protein and Preparation of a Monoclonal Antibody.

Monoclonal antibodies in immunodiagnosis and immunotherapy
Hepatitis C virus (HCV) is a significant health threat that has been extensively investigated worldwide. Improving the sensitivity and specificity of laboratory tests for screening and early diagnosis of HCV in a relevant population is an effective m...

[Effect of Recombinant Antigen Ferritin on DC Induce Immune Response].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
OBJECTIVE: To explore the immunoreaction against protoscolex infection by the recombinant ferritin of echinococcus granulosus (rEg.ferritin) immunized mice.

Cost-effective batch production process of scFv antibody in Escherichia coli.

Human antibodies
BACKGROUND: Cost-effective production of antibody (Ab) fragments is of great interests of many pharmaceutical industries, in large part due to their high usages in research, diagnosis and therapy. Thus, the production of Abs necessitates accomplishme...

Medium Optimization for Recombinant Soluble Arginine Deiminase Expression in Escherichia coli Using Response Surface Methodology.

Current pharmaceutical biotechnology
OBJECTIVE: Optimization of the medium for recombinant arginine deiminase production in E. coli was performed using response surface methodology. This is the first study of optimization of recombinant arginine deiminase production in E. coli by the us...

[Gene optimization and efficient expression of Trichoderma reesei Cel5A in Pichia pastoris].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Deficient activity of endo-1,4-beta-glucanase II (Cel5A) secreted by Trichoderma reesei is one of the challenges involved in effective cellulase saccharification of cellulosic substrates. Therefore, we expressed Cel5A in Pichia pastoris by constructi...

[Process Optimization of PEGylating Fused Protein of LL-37 and Interferon-α2a].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
PEGylating is an effective way for prolonging the half-time period and decreasing the immunogenicity of protein drugs. With experiments of single factor, it was proved that the optimal processes for PEGylating the fused protein of LL-37 and interfero...

[Cloning, expression, and purification of c-di-AMP metabolism-related genes from Porphyromonas gingivalis].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
OBJECTIVE: To clone, express, and purify cyclic diadenosine monophosphate (c-di-AMP) metabolism-related genes from Porphyromonas gingivalis (P. gingivalis) ATCC33277.