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Second Harmonic Generation Microscopy

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Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy.

eLife
Three-photon excitation has recently been demonstrated as an effective method to perform intravital microscopy in deep, previously inaccessible regions of the mouse brain. The applicability of 3-photon excitation for deep imaging of other, more heter...

Fast intraoperative histology-based diagnosis of gliomas with third harmonic generation microscopy and deep learning.

Scientific reports
Management of gliomas requires an invasive treatment strategy, including extensive surgical resection. The objective of the neurosurgeon is to maximize tumor removal while preserving healthy brain tissue. However, the lack of a clear tumor boundary h...

Improved segmentation of collagen second harmonic generation images with a deep learning convolutional neural network.

Journal of biophotonics
Collagen fibers play an important role in both the structure and function of various tissues in the human body. Visualization and quantitative measurements of collagen fibers are possible through imaging modalities such as second harmonic generation ...

Rapid and Precise Diagnosis of Retroperitoneal Liposarcoma with Deep-Learned Label-Free Molecular Microscopy.

Analytical chemistry
The retroperitoneal liposarcoma (RLPS) is a rare malignancy whose only curative therapy is surgical resection. However, well-differentiated liposarcomas (WDLPSs), one of its most common types, can hardly be distinguished from normal fat during operat...

Ovarian cancer identification technology based on deep learning and second harmonic generation imaging.

Journal of biophotonics
Ovarian cancer is among the most common gynecological cancers and the eighth leading cause of cancer-related deaths among women worldwide. Surgery is among the most important options for cancer treatment. During surgery, a biopsy is generally require...

Machine learning-assisted mid-infrared spectrochemical fibrillar collagen imaging in clinical tissues.

Journal of biomedical optics
SIGNIFICANCE: Label-free multimodal imaging methods that can provide complementary structural and chemical information from the same sample are critical for comprehensive tissue analyses. These methods are specifically needed to study the complex tum...

Automated Detection of Microcracks Within Second Harmonic Generation Images of Cartilage Using Deep Learning.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Articular cartilage, essential for smooth joint movement, can sustain micrometer-scale microcracks in its collagen network from low-energy impacts previously considered non-injurious. These microcracks may propagate under cyclic loading, impairing ca...