AIMC Topic: Microscopy, Fluorescence, Multiphoton
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Quantitative image analysis of the extracellular matrix of esophageal squamous cell carcinoma and high grade dysplasia via two-photon microscopy.
Scientific reports
Aug 7, 2025
Squamous cell carcinoma (SCC) and high-grade dysplasia (HGD) are two different pathological entities; however, they sometimes share similarities in histological structure depending on the context. Thus, distinguishing between the two may require care...
Comparison of conventional and radiomics-based analysis of myocardial infarction using multimodal non-linear optical microscopy.
Scientific reports
Jul 18, 2025
Myocardial infarction, a leading cause of mortality worldwide, leaves survivors at significant risk of recurrence caused by scar-related re-entrant ventricular tachyarrhythmias. Effective treatment with ablation therapy requires a precise guidance sy...
Multimodal Optical Imaging Combined with Radiomic Analysis for Fibrotic Cardiac Tissue Investigation.
Analytical chemistry
Jul 5, 2025
Understanding the process of fibrotic scarring of the myocardium is critical for the diagnosis and risk stratification of life-threatening cardiac dysfunction. Complex changes in structure, composition, and conductivity occurring at different stages ...
Deep structural brain imaging via computational three-photon microscopy.
Journal of biomedical optics
Mar 29, 2025
SIGNIFICANCE: High-resolution optical imaging at significant depths is challenging due to scattering, which impairs image quality in living matter with complex structures. We address the need for improved imaging techniques in deep tissues.
A first explainable-AI-based workflow integrating forward-forward and backpropagation-trained networks of label-free multiphoton microscopy images to assess human biopsies of rare neuromuscular disease.
Computer methods and programs in biomedicine
Mar 21, 2025
BACKGROUND AND OBJECTIVE: Diagnosis of rare neuromuscular diseases often relies on muscle biopsy analysis, which varies based on the evaluator's experience. Advances in deep learning show promise in improving diagnostic accuracy by identifying standa...
Fast In Vivo Two-Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self-Supervised Learning.
Journal of biophotonics
Feb 5, 2025
Two-photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF imaging systems to simultaneously achieve high resolution and speed. In this work, we de...
Diagnosis of Fibrotic Interstitial Lung Diseases Based on the Combination of Label-Free Quantitative Multiphoton Fiber Histology and Machine Learning.
Laboratory investigation; a journal of technical methods and pathology
Dec 13, 2024
Interstitial lung disease (ILD), characterized by inflammation and fibrosis, often suffers from low diagnostic accuracy and consistency. Traditional hematoxylin and eosin (H&E) staining primarily reveals cellular inflammation with limited detail on f...
Measuring Metabolic Changes in Cancer Cells Using Two-Photon Fluorescence Lifetime Imaging Microscopy and Machine-Learning Analysis.
Journal of biophotonics
Nov 25, 2024
Two-photon (2P) fluorescence lifetime imaging microscopy (FLIM) was used to track cellular metabolism with drug treatment of auto-fluorescent coenzymes NAD(P)H and FAD in living cancer cells. Simultaneous excitation at 800 nm of both coenzymes was co...
AutoLNMNet: Automated Network for Estimating Lymph-Node Metastasis in EGC Using a Pyramid Vision Transformer and Data Derived From Multiphoton Microscopy.
Microscopy research and technique
Oct 1, 2024
Lymph-node status is important in decision-making during early gastric cancer (EGC) treatment. Currently, endoscopic submucosal dissection is the mainstream treatment for EGC. However, it is challenging for even experienced endoscopists to accurately...
AI-based digital pathology provides newer insights into lifestyle intervention-induced fibrosis regression in MASLD: An exploratory study.
Liver international : official journal of the International Association for the Study of the Liver
Jul 4, 2024
BACKGROUND AND AIMS: Lifestyle intervention is the mainstay of therapy for metabolic dysfunction-associated steatohepatitis (MASH), and liver fibrosis is a key consequence of MASH that predicts adverse clinical outcomes. The placebo response plays a ...
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