AIMC Topic: HEK293 Cells

Clear Filters Showing 21 to 30 of 73 articles

Easy-Prime: a machine learning-based prime editor design tool.

Genome biology Aug 19, 2021
Prime editing is a revolutionary genome-editing technology that can make a wide range of precise edits in DNA. However, designing highly efficient prime editors (PEs) remains challenging. We develop Easy-Prime, a machine learning-based program traine...
Humans Machine Learning DNA CRISPR-Cas Systems HEK293 Cells Gene Editing RNA, Guide, CRISPR-Cas Systems
View on PubMed DOI

Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods.

Nature communications Aug 12, 2021
Efficient and precise base editors (BEs) for C-to-G transversion are highly desirable. However, the sequence context affecting editing outcome largely remains unclear. Here we report engineered C-to-G BEs of high efficiency and fidelity, with the seq...
Animals Mice Escherichia coli CRISPR-Cas Systems Humans Machine Learning Female Reproducibility of Results Binding Sites Codon Gene Library Caenorhabditis elegans Cytidine Deaminase HEK293 Cells Uracil-DNA Glycosidase Base Sequence Gene Editing
View on PubMed DOI

A deep learning approach to identify gene targets of a therapeutic for human splicing disorders.

Nature communications Jun 7, 2021
Pre-mRNA splicing is a key controller of human gene expression. Disturbances in splicing due to mutation lead to dysregulated protein expression and contribute to a substantial fraction of human disease. Several classes of splicing modulator compound...
Humans Computational Biology Mutation Phenethylamines HEK293 Cells Animals Transcriptome Deep Learning Mice Gene Targeting Cystic Fibrosis Transmembrane Conductance Regulator RNA Splicing Exons tau Proteins MutL Protein Homolog 1 Sterol Esterase Pyridazines Mice, Transgenic
View on PubMed DOI

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning.

Nature communications May 28, 2021
The design of CRISPR gRNAs requires accurate on-target efficiency predictions, which demand high-quality gRNA activity data and efficient modeling. To advance, we here report on the generation of on-target gRNA activity data for 10,592 SpCas9 gRNAs. ...
Lentivirus Genetic Vectors RNA, Guide, CRISPR-Cas Systems CRISPR-Cas Systems HEK293 Cells Plasmids Gene Editing Humans Software Deep Learning Computational Biology
View on PubMed DOI

An Integrated Approach toward NanoBRET Tracers for Analysis of GPCR Ligand Engagement.

Molecules (Basel, Switzerland) May 12, 2021
Gaining insight into the pharmacology of ligand engagement with G-protein coupled receptors (GPCRs) under biologically relevant conditions is vital to both drug discovery and basic research. NanoLuc-based bioluminescence resonance energy transfer (Na...
HEK293 Cells Luminescent Agents Binding, Competitive Bioluminescence Resonance Energy Transfer Techniques Receptors, G-Protein-Coupled Luciferases Transfection Ligands Humans Machine Learning Molecular Docking Simulation Drug Discovery Protein Binding
View on PubMed DOI

Identification of SARS-CoV-2 viral entry inhibitors using machine learning and cell-based pseudotyped particle assay.

Bioorganic & medicinal chemistry Mar 26, 2021
In response to the pandemic caused by SARS-CoV-2, we constructed a hybrid support vector machine (SVM) classification model using a set of publicly posted SARS-CoV-2 pseudotyped particle (PP) entry assay repurposing screen data to identify novel pote...
Support Vector Machine Drug Repositioning Area Under Curve HEK293 Cells SARS-CoV-2 Databases, Chemical Microbial Sensitivity Tests Small Molecule Libraries ROC Curve Humans Virus Internalization Antiviral Agents
View on PubMed DOI

Screening of a novel free fatty acid receptor 1 (FFAR1) agonist peptide by phage display and machine learning based-amino acid substitution.

Biochemical and biophysical research communications Mar 9, 2021
Free fatty acid receptor 1 (FFAR1 or GPR40) has attracted attention for the treatment of type 2 diabetes mellitus, and various small-molecule agonists have been developed. However, most FFAR1 agonists as well as endogenous ligands, such as linoleic a...
Humans Glucose Peptide Library Cloning, Molecular Machine Learning Drug Evaluation, Preclinical Cell Line Amino Acid Substitution Amino Acid Sequence Peptides Regression Analysis Transforming Growth Factor alpha HEK293 Cells Insulin Receptors, G-Protein-Coupled Protein Binding
View on PubMed DOI

HSM6AP: a high-precision predictor for the Homo N6-methyladenosine (m^6 A) based on multiple weights and feature stitching.

RNA biology Feb 12, 2021
Recent studies have shown that RNA methylation modification can affect RNA transcription, metabolism, splicing and stability. In addition, RNA methylation modification has been associated with cancer, obesity and other diseases. Based on information ...
HEK293 Cells Gene Expression Regulation Adenosine Computational Biology A549 Cells Humans HeLa Cells Machine Learning Methylation Algorithms
View on PubMed DOI

Systematic characterization of mutations altering protein degradation in human cancers.

Molecular cell Feb 9, 2021
The ubiquitin-proteasome system (UPS) is the primary route for selective protein degradation in human cells. The UPS is an attractive target for novel cancer therapies, but the precise UPS genes and substrates important for cancer growth are incomple...
Models, Genetic Neoplasm Proteins Mutation HEK293 Cells Humans Neoplasms Deep Learning Cell Line, Tumor Proteolysis
View on PubMed DOI

sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks.

Plant molecular biology Jan 1, 2021
We proposed an ensemble convolutional neural network model to identify sgRNA high on-target activity in four crops and we used one-hot encoding and k-mers for sequence encoding. As an important component of the CRISPR/Cas9 system, single-guide RNA (s...
HeLa Cells Sorghum Zea mays Humans Crops, Agricultural CRISPR-Cas Systems Gene Editing Neural Networks, Computer HCT116 Cells Triticum Glycine max Algorithms HEK293 Cells Internet RNA, Guide, CRISPR-Cas Systems Computational Biology
View on PubMed DOI
Popular Topics
Recent Journals