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Microfluidic Analytical Techniques

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High-Quality Immunohistochemical Stains Through Computational Assay Parameter Optimization.

IEEE transactions on bio-medical engineering
Accurate profiling of tumors using immunohistochemistry (IHC) is essential in cancer diagnosis. The inferences drawn from IHC-stained images depend to a great extent on the quality of immunostaining, which is in turn affected strongly by assay parame...

A new method using machine learning for automated image analysis applied to chip-based digital assays.

The Analyst
Chip-based digital assays such as the digital polymerase chain reaction (digital PCR), digital loop-mediated amplification (digital LAMP), digital enzyme-linked immunosorbent assay (digital ELISA) and digital proximity ligation assay (digital PLA) ne...

Automated System for Small-Population Single-Particle Processing Enabled by Exclusive Liquid Repellency.

SLAS technology
Exclusive liquid repellency (ELR) describes an extreme wettability phenomenon in which a liquid phase droplet is completely repelled from a solid phase when exposed to a secondary immiscible liquid phase. Earlier, we developed a multi-liquid-phase op...

Two-Dimensional Light Scattering Anisotropy Cytometry for Label-Free Classification of Ovarian Cancer Cells via Machine Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
We develop a single-mode fiber-based cytometer for the obtaining of two-dimensional (2D) light scattering patterns from static single cells. Anisotropy of the 2D light scattering patterns of single cells from ovarian cancer and normal cell lines is i...

CD4+ versus CD8+ T-lymphocyte identification in an integrated microfluidic chip using light scattering and machine learning.

Lab on a chip
T lymphocytes are a group of cells representing the main effectors of human adaptive immunity. Characterization of the most representative T-lymphocyte subclasses, CD4+ and CD8+, is challenging, but has a significant impact on clinical decisions. Up ...

Genome-scale transcriptional dynamics and environmental biosensing.

Proceedings of the National Academy of Sciences of the United States of America
Genome-scale technologies have enabled mapping of the complex molecular networks that govern cellular behavior. An emerging theme in the analyses of these networks is that cells use many layers of regulatory feedback to constantly assess and precisel...

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications.

Molecules (Basel, Switzerland)
Flow chemistry is an area of contemporary chemistry exploiting the hydrodynamic conditions of flowing liquids to provide particular environments for chemical reactions. These particular conditions of enhanced and strictly regulated transport of reage...

A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry.

Analytical and bioanalytical chemistry
Microfluidic applications such as active particle sorting or selective enrichment require particle classification techniques that are capable of working in real time. In this paper, we explore the use of neural networks for fast label-free particle c...

Intelligent classification of platelet aggregates by agonist type.

eLife
Platelets are anucleate cells in blood whose principal function is to stop bleeding by forming aggregates for hemostatic reactions. In addition to their participation in physiological hemostasis, platelet aggregates are also involved in pathological ...