AIMC Topic: Nucleic Acid Amplification Techniques
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Assessing the performance of a point-of-need diagnostic algorithm in rapid detection of peripheral lymph node tuberculosis (Mobile-TB-Lab): a diagnostic evaluation study protocol.
BMJ open
May 30, 2025
INTRODUCTION: Early and accurate diagnosis of tuberculosis (TB) is central to ensuring the proper treatment and curbing the transmission of the disease. Despite the significant burden, the diagnosis of peripheral lymph node(LN)TB, the most prevalent ...
A drop dispenser for simplifying on-farm detection of foodborne pathogens.
PloS one
Dec 31, 2024
Nucleic-acid biosensors have emerged as useful tools for on-farm detection of foodborne pathogens on fresh produce. Such tools are specifically designed to be user-friendly so that a producer can operate them with minimal training and in a few simple...
Artificial intelligence-driven quantification of antibiotic-resistant Bacteria in food by color-encoded multiplex hydrogel digital LAMP.
Food chemistry
Dec 4, 2024
Antibiotic-resistant bacteria pose considerable risks to global health, particularly through transmission in the food chain. Herein, we developed the artificial intelligence-driven quantification of antibiotic-resistant bacteria in food using a color...
AI-empowered visualization of nucleic acid testing.
Life sciences
Oct 31, 2024
AIMS: The visualization of nucleic acid testing (NAT) results plays a critical role in diagnosing and monitoring infectious and genetic diseases. The review aims to review the current status of AI-based NAT result visualization. It systematically int...
A novel machine-learning aided platform for rapid detection of urine ESBLs and carbapenemases: URECA-LAMP.
Journal of clinical microbiology
Oct 24, 2024
Pathogenic gram-negative bacteria frequently carry genes encoding extended-spectrum beta-lactamases (ESBL) and/or carbapenemases. Of great concern are carbapenem resistant , , and . Despite the need for rapid AMR diagnostics globally, current molecu...
High-Throughput and Integrated CRISPR/Cas12a-Based Molecular Diagnosis Using a Deep Learning Enabled Microfluidic System.
ACS nano
Aug 22, 2024
CRISPR/Cas-based molecular diagnosis demonstrates potent potential for sensitive and rapid pathogen detection, notably in SARS-CoV-2 diagnosis and mutation tracking. Yet, a major hurdle hindering widespread practical use is its restricted throughput,...
Deep Learning Enabled Universal Multiplexed Fluorescence Detection for Point-of-Care Applications.
ACS sensors
Jul 15, 2024
There is a significant demand for multiplexed fluorescence sensing and detection across a range of applications. Yet, the development of portable and compact multiplexable systems remains a substantial challenge. This difficulty largely stems from th...
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning.
Journal of visualized experiments : JoVE
May 10, 2024
We report a fast, easy-to-implement, highly sensitive, sequence-specific, and point-of-care (POC) DNA virus detection system, which combines recombinase polymerase amplification (RPA) and CRISPR/Cas12a system for trace detection of DNA viruses. Targe...
Nucleic Acid Plate Culture: Label-Free and Naked-Eye-Based Digital Loop-Mediated Isothermal Amplification in Hydrogel with Machine Learning.
ACS sensors
Apr 11, 2024
Digital nucleic acid amplification enables the absolute quantification of single molecules. However, due to the ultrasmall reaction volume in the digital system (, short light path), most digital systems are limited to fluorescence signals, while lab...
Mesophilic Argonaute-Mediated Polydisperse Droplet Biosensor for Amplification-Free, One-Pot, and Multiplexed Nucleic Acid Detection Using Deep Learning.
Analytical chemistry
Jan 23, 2024
Detection of nucleic acids from a single multiplexed and amplification-free test is critical for ensuring food safety, clinical diagnostics, and environmental monitoring. In this study, we introduced a mesophilic Argonaute protein from (CbAgo), whic...
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