AIMC Topic: Oocytes

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Robotic Immobilization of Motile Sperm for Clinical Intracytoplasmic Sperm Injection.

IEEE transactions on bio-medical engineering
OBJECTIVE: In clinical intracytoplasmic sperm injection (ICSI), a motile sperm must be immobilized before insertion into an oocyte. This paper aims to develop a robotic system for automated tracking, orientation control, and immobilization of motile ...

A deep learning framework to discern and count microscopic nematode eggs.

Scientific reports
In order to identify and control the menace of destructive pests via microscopic image-based identification state-of-the art deep learning architecture is demonstrated on the parasitic worm, the soybean cyst nematode (SCN), Heterodera glycines. Soybe...

Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro.

Theriogenology
This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated sec...

In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption.

Theriogenology
The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicle...

Follicle-stimulating hormone-induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes.

Domestic animal endocrinology
The effect of follicle-stimulating hormone (FSH) on apoptotic status of cumulus cells, expression of proapoptotic and antiapoptotic genes, and development rate of in vitro fertilization-produced buffalo embryos were investigated. FSH supplementation ...

Exposure to Aroclor-1254 impairs spindle assembly during mouse oocyte maturation.

Environmental toxicology
Polychlorinated biphenyls (PCBs), as typical environmental estrogen disruptors, are a structurally-related group of halogenated aromatic hydrocarbons that are composed of 209 isomers and present as a mixture in the environment. PCBs congener with dif...

Deep-Learning Based Quantification of Bovine Oocyte Quality From Microscopy Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
The success rate of bovine in vitro embryo reproduction is low and highly dependent on the oocyte quality. The selection of the oocyte to be fertilized is done by the embryologists' visual examination of oocytes. It is time-consuming, subjective, and...

Impact of oocyte donor age and breed on embryo production in cattle, and relationship of dairy and beef embryo recipients on pregnancy and the subsequent performance of offspring: A review.

Reproduction, fertility, and development
Genomic selection combined with in vitro embryo production (IVEP) with oocytes from heifer calves provides a powerful technology platform to reduce generation interval and significantly increase the rate of genetic gain in cattle. The ability to obta...

Parameters to identify good quality oocytes and embryos in cattle.

Reproduction, fertility, and development
Oocyte/embryo selection methodologies are either invasive or noninvasive and can be applied at various stages of development from the oocyte to cleaved embryos and up to the blastocyst stage. Morphology and the proportion of embryos developing to the...

Follicular fluid supplemented with copper and zinc increase the maturation rate of mouse vitrified-warmed oocytes.

Cryo letters
BACKGROUND: Immature oocyte cryopreservation is a therapeutic option in assisted reproductive technology.