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Recombinant Proteins

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[Construction and high-density fermentation of alkaline pectate lyase high-yield yeast].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Pectate lyase is widely applied in ramie degumming and fabric bioscouring in the textile industry. Compared to conventional processes that involve high alkaline and high temperature treatment, enzyme based treatments have significant advantages in fi...

Prokaryotic Expression of Hepatitis C Virus-NS3 Protein and Preparation of a Monoclonal Antibody.

Monoclonal antibodies in immunodiagnosis and immunotherapy
Hepatitis C virus (HCV) is a significant health threat that has been extensively investigated worldwide. Improving the sensitivity and specificity of laboratory tests for screening and early diagnosis of HCV in a relevant population is an effective m...

[Effect of Recombinant Antigen Ferritin on DC Induce Immune Response].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
OBJECTIVE: To explore the immunoreaction against protoscolex infection by the recombinant ferritin of echinococcus granulosus (rEg.ferritin) immunized mice.

[Gene optimization and efficient expression of Trichoderma reesei Cel5A in Pichia pastoris].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Deficient activity of endo-1,4-beta-glucanase II (Cel5A) secreted by Trichoderma reesei is one of the challenges involved in effective cellulase saccharification of cellulosic substrates. Therefore, we expressed Cel5A in Pichia pastoris by constructi...

Expression of rabies virus glycoprotein in the methylotrophic yeast Pichia pastoris.

Biotechnology and applied biochemistry
Rabies is a fatal disease that can be prevented by vaccination. Different approaches were investigated to develop novel human rabies vaccines with improved features compared to the current available vaccines, among them is the use of heterologous gen...

Optimization of an Anti-poly(ethylene glycol) (anti-PEG) Cell-Based Capture System To Quantify PEG and PEGylated Molecules.

Analytical chemistry
Sensitive determination of the pharmacokinetics of PEGylated molecules can accelerate the process of drug development. Here, we combined different anti-PEG Fab expressing 293T cells as capture cells (293T/3.3, 293T/6.3, and 293T/15-2b cells) with fou...

Efficacy and Safety Evaluation on Arterial Thrombolysis in Treating Acute Cerebral Infarction.

Cell biochemistry and biophysics
The objective of this study was to evaluate the efficacy and safety of intra-arterial thrombolysis in treating acute cerebral infarction and further discuss the indications of acute cerebral infarction treatment, in order to enhance the therapeutic e...

A robust robotic high-throughput antibody purification platform.

Journal of chromatography. A
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...

Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning.

Biochemistry
As one of the most intrinsically reactive amino acids, cysteine carries a variety of important biochemical functions, including catalysis and redox regulation. Discovery and characterization of cysteines with heightened reactivity will help annotate ...