AIMC Topic: Trypsin

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Quantitative ultrasound classification of healthy and chemically degraded ex-vivo cartilage.

Scientific reports Jul 1, 2025
In this study, we explore the potential of ten quantitative (radiofrequency-based) ultrasound parameters to assess the progressive loss of collagen and proteoglycans, mimicking an osteoarthritis condition in ex-vivo bovine cartilage samples. Most ana...
Ultrasonography Cattle Proteoglycans Cartilage Trypsin Cartilage, Articular Osteoarthritis Collagenases Collagen Machine Learning Animals
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Refinement of paramagnetic bead-based digestion protocol for automatic sample preparation using an artificial neural network.

Talanta Mar 23, 2024
Despite technological advances in the proteomics field, sample preparation still represents the main bottleneck in mass spectrometry (MS) analysis. Bead-based protein aggregation techniques have recently emerged as an efficient, reproducible, and hig...
HeLa Cells Humans Mice Automation Neural Networks, Computer Trypsin Rats Animals Proteomics
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Interpretable Machine Learning of Amino Acid Patterns in Proteins: A Statistical Ensemble Approach.

Journal of chemical theory and computation Aug 8, 2023
Explainable and interpretable unsupervised machine learning helps one to understand the underlying structure of data. We introduce an ensemble analysis of machine learning models to consolidate their interpretation. Its application shows that restric...
Machine Learning Peptide Fragments Amino Acids Amino Acid Sequence Trypsin
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Prognostic assessment capability of a five-gene signature in pancreatic cancer: a machine learning based-study.

BMC gastroenterology Mar 11, 2023
BACKGROUND: A prognostic assessment method with good sensitivity and specificity plays an important role in the treatment of pancreatic cancer patients. Finding a way to evaluate the prognosis of pancreatic cancer is of great significance for the tre...
Humans Machine Learning Prognosis ROC Curve Pancreatic Neoplasms Trypsin
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History Dependence in a Chemical Reaction Network Enables Dynamic Switching.

Small (Weinheim an der Bergstrasse, Germany) Mar 8, 2022
This work describes an enzymatic autocatalytic network capable of dynamic switching under out-of-equilibrium conditions. The network, wherein a molecular fuel (trypsinogen) and an inhibitor (soybean trypsin inhibitor) compete for a catalyst (trypsin)...
Catalysis Trypsin Neural Networks, Computer
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Investigation and Highly Accurate Prediction of Missed Tryptic Cleavages by Deep Learning.

Journal of proteome research Jun 17, 2021
Trypsin is one of the most important and widely used proteolytic enzymes in mass spectrometry (MS)-based proteomic research. It exclusively cleaves peptide bonds at the C-terminus of lysine and arginine. However, the cleavage is also affected by seve...
Peptides Deep Learning Proteomics Mass Spectrometry Trypsin
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AlphaFold - A Personal Perspective on the Impact of Machine Learning.

Journal of molecular biology Jun 2, 2021
I outline how over my career as a protein scientist Machine Learning has impacted my area of science and one of my pastimes, chess, where there are some interesting parallels. In 1968, modelling of three-dimensional structures was initiated based on ...
Protein Conformation Trypsin Chymotrypsin Protein Folding Animals Proteins Models, Molecular Machine Learning Software Humans
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Extraction, identification and structure-activity relationship of antioxidant peptides from sesame (Sesamum indicum L.) protein hydrolysate.

Food research international (Ottawa, Ont.) Sep 4, 2018
To elucidate the sequence, origin and structure-activity relationship of antioxidant peptides from sesame protein, sesame protein was hydrolysed by a dual-enzyme system comprised alcalase and trypsin, then this hydrolysate was fractionated by ultrafi...
Sulfonic Acids Picrates Models, Molecular Benzothiazoles Trypsin Antioxidants Amino Acid Sequence Oligopeptides Sesamum Seeds Biphenyl Compounds Quantitative Structure-Activity Relationship Subtilisins Protein Hydrolysates
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Protease inhibitors in various flours and breads: Effect of fermentation, baking and in vitro digestion on trypsin and chymotrypsin inhibitory activities.

Food chemistry Dec 18, 2016
In this study trypsin (TIA) and chymotrypsin inhibitory (CIA) activities were determined in the extracts of wheat, rye mix, mixed cereals and, whole wheat flours and, breads made with these flours. In addition, effects of fermentation, baking and in ...
Triticum Edible Grain Fermentation Digestion Cooking Bread Protease Inhibitors Trypsin Chymotrypsin Flour Secale
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Ultrasensitive Protease Sensors Using Selective Affinity Binding, Selective Proteolytic Reaction, and Proximity-Dependent Electrochemical Reaction.

Analytical chemistry Nov 30, 2016
The development of a fast and ultrasensitive protease detection method is a challenging task. This paper reports ultrasensitive protease sensors exploiting (i) selective affinity binding, (ii) selective proteolytic reaction, and (iii) proximity-depen...
Immunoglobulin G Trypsin Oxides Proteolysis Electrodes Oxidation-Reduction Oligopeptides Aminophenols Graphite Electrochemical Techniques Tin Compounds Limit of Detection
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