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Aptamers, Nucleotide

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In vitro selection of DNA aptamers and their integration in a competitive voltammetric biosensor for azlocillin determination in waste water.

Analytica chimica acta
The uncontrolled usage of veterinary antibiotics has led to their widespread pollution in waterways and milk products. Potential impact of antibiotic residues on the environment and human health such as increased antibiotic resistance of microorganis...

An antifouling peptide-based biosensor for determination of Streptococcus pneumonia markers in human serum.

Biosensors & bioelectronics
We report a peptide-based sensor that involves a multivalent interaction with L-ascorbate 6-phosphate lactonase (UlaG), a protein marker of Streptococcus pneumonia. By integrating the antifouling feature of the sensor, we significantly improved the s...

Employing AgNPs doped amidoxime-modified polyacrylonitrile (PAN-oxime) nanofibers for target induced strand displacement-based electrochemical aptasensing of CA125 in ovarian cancer patients.

Materials science & engineering. C, Materials for biological applications
In this study, a high-performance biosensing nanoplatform based on amidoxime-modified polyacrylonitrile nanofibers decorated with Ag nanoparticles (AgNPs-PAN-oxime NFs) is described. The AgNPs-PAN-oxime NFs were prepared by the combination of electro...

Focusing aptamer selection on the glycan structure of prostate-specific antigen: Toward more specific detection of prostate cancer.

Biosensors & bioelectronics
The development of chemical sensors capable of detecting the specific glycosylation patterns of proteins offers a powerful mean for the early detection of cancer. Unfortunately, this strategy is scarcely explored because receptors recognizing the gly...

Tuning the Performance of Synthetic Riboswitches using Machine Learning.

ACS synthetic biology
Riboswitch development for clinical, technological, and synthetic biology applications constantly seeks to optimize regulatory behavior. Here, we present a machine learning approach to improve the regulation of a tetracycline (tc)-dependent riboswitc...

Prediction of aptamer-protein interacting pairs based on sparse autoencoder feature extraction and an ensemble classifier.

Mathematical biosciences
Aptamer-protein interacting pairs play important roles in physiological functions and structural characterization. Identifying aptamer-protein interacting pairs is challenging and limited, despite of the tremendous applications of aptamers. Therefore...

Robotic assisted generation of 2'-deoxy-2'-fluoro-modifed RNA aptamers - High performance enabling strategies in aptamer selection.

Methods (San Diego, Calif.)
Aptamer selection is a laborious procedure, requiring expertise and significant resources. These characteristics limit the accessibility of researchers to these molecular tools. We describe a selection procedure, making use of a robotic system that a...

An Intelligent DNA Nanorobot with Enhanced Protein Lysosomal Degradation of HER2.

Nano letters
DNA nanorobots have emerged as new tools for nanomedicine with the potential to ameliorate the delivery and anticancer efficacy of various drugs. DNA nanostructures have been considered one of the most promising nanocarriers. In the present study, we...

Constructive Prediction of Potential RNA Aptamers for a Protein Target.

IEEE/ACM transactions on computational biology and bioinformatics
Aptamers are short single-stranded nucleic acids that bind to target molecules with high affinity and selectivity. Aptamers are generally identified in vitro by performing SELEX (systematic evolution of ligands by exponential enrichment). Complementi...

A generative model for constructing nucleic acid sequences binding to a protein.

BMC genomics
BACKGROUND: Interactions between protein and nucleic acid molecules are essential to a variety of cellular processes. A large amount of interaction data generated by high-throughput technologies have triggered the development of several computational...