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Aptamers, Nucleotide

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Aptamer based fluorometric β-lactoglobulin assay based on the use of magnetic nanoparticles and carbon dots.

Mikrochimica acta
The authors describe a fluorometric aptamer based assay for detecting β-lactoglobulin by using carbon dots (C-dots) as a signal indicator. The aptamer was immoblized on magnetite (FeO) nanoparticles (MNPs), and the C-dots served as a label for the co...

Salinomycin-loaded lipid-polymer nanoparticles with anti-CD20 aptamers selectively suppress human CD20+ melanoma stem cells.

Acta pharmacologica Sinica
Melanoma is the deadliest type of skin cancer. CD20+ melanoma stem cells (CSCs) are pivotal for metastasis and initiation of melanoma. Therefore, selective elimination of CD20+ melanoma CSCs represents an effective treatment to eradicate melanoma. Sa...

Highly sensitive colorimetric aptasensor for ochratoxin A detection based on enzyme-encapsulated liposome.

Analytica chimica acta
A simple, low-cost, and sensitive liposome-based colorimetric aptasensor has been developed to detect ochratoxin A (OTA). Specifically, a dumbbell-shaped probe was designed, including magnetic beads (MBs), double-stranded DNA (dsDNA), and enzyme-enca...

Aptamer facilitated purification of functional proteins.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
DNA aptamers are attractive capture probes for affinity chromatography since, in contrast to antibodies, they can be chemically synthesized and, in contrast to tag-specific capture probes (such as Nickel-NTA or Glutathione), they can be used for puri...

A sensitive assay based on specific aptamer binding for the detection of Salmonella enterica serovar Typhimurium in milk samples by microchip capillary electrophoresis.

Journal of chromatography. A
The detection of Salmonella enterica serovar Typhimurium (S. Typhimurium) is very important for the prevention of food poisoning and other infectious diseases. Here we reported a simple and sensitive strategy to test S. Typhimurium by microchip capil...

Ultrasensitive flexible FET-type aptasensor for CA 125 cancer marker detection based on carboxylated multiwalled carbon nanotubes immobilized onto reduced graphene oxide film.

Analytica chimica acta
The development of a novel flexible and ultrasensitive aptasensor based on carboxylated multiwalled carbon nanotubes (MWCNTs)/ reduced graphene oxide-based field effect transistor (FET) has been reported for label-free detection of the ovarian cancer...

Fluorescence resonance energy transfer aptasensor between nanoceria and graphene quantum dots for the determination of ochratoxin A.

Analytica chimica acta
In the present work, colloidal cerium oxide nanoparticles (nanoceria) and graphene quantum dots (GQDs) were firstly synthesized by sol-gel method and pyrolysis respectively, which all have a uniform nano-size and significant fluorescence emission. Du...

An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR.

Talanta
Chloramphenicol (CAP) residues can not only harm human health through entering food chain, but also cause the spreading of drug-resistant bacteria, thereby leading to secondary environmental pollution. Therefore, it is in urgent need of establishing ...

Synthetic Ion Channels and DNA Logic Gates as Components of Molecular Robots.

Chemphyschem : a European journal of chemical physics and physical chemistry
A molecular robot is a next-generation biochemical machine that imitates the actions of microorganisms. It is made of biomaterials such as DNA, proteins, and lipids. Three prerequisites have been proposed for the construction of such a robot: sensors...

Smart Plasmonic Nanorobot for Real-Time Monitoring Cytochrome c Release and Cell Acidification in Apoptosis during Electrostimulation.

Analytical chemistry
Cytochrome c (Cyt c) release and cellular pH change are two important mediators of apoptosis. Effective methods to regulate or monitor such two events are therefore highly desired for apoptosis research and cancer cell therapy. Herein, we exploited e...