AIMC Topic: Bacterial Proteins

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Structural and functional analysis of the accessory gene regulators of Staphylococcus aureus and Staphylococcus epidermidis: an in Silico approach.

BMC microbiology
BACKGROUND: Staphylococcus aureus and Staphylococcus epidermidis are tenacious pathogens that cause toxic shock syndrome. Accessory gene regulator (Agr) of Staphylococcus sp. controls the expression of multiple genes that encode virulence properties....

Discovery of CRISPR-Cas12a clades using a large language model.

Nature communications
CRISPR-Cas systems revolutionize life science. Metagenomes contain millions of unknown Cas proteins. Traditional mining relies on protein sequence alignments. In this work, we employ an evolutionary scale language model (ESM) to learn the information...

Integrative machine learning and RT-qPCR analysis identify key stress-responsive genes in Thermus thermophilus HB8.

Genetica
Bacteria are constantly exposed to diverse environmental stresses, necessitating complex adaptive mechanisms for survival. Thermus thermophilus, a thermophilic extremophile, serves as an excellent model for investigating these responses due to its re...

Computational function prediction of bacteria and phage proteins.

Microbiology and molecular biology reviews : MMBR
SUMMARYUnderstanding protein functions is crucial for interpreting microbial life; however, reliable function annotation remains a major challenge in computational biology. Despite significant advances in bioinformatics methods, ~30% of all bacterial...

LC-MS/MS metabolomics unravels the resistant phenotype of carbapenemase-producing Enterobacterales.

Metabolomics : Official journal of the Metabolomic Society
INTRODUCTION: The degree of antimicrobial resistance demonstrated by carbapenemase-producing Enterobacterales (CPE) represents a growing public health challenge. Conventional methods for detecting CPE involve culture-based techniques with lengthy inc...

A computational pipeline for predicting distal hotspots in an artificial enzyme.

International journal of biological macromolecules
Targeting distal mutations holds promising implications for enzyme engineering. Here, we present an open-source computational workflow designed to explore the functional impact of distal sites, demonstrated on an artificial enzyme built on the widely...

Machine Learning and DIA Proteomics Reveal New Insights into Carbapenem Resistance Mechanisms in .

Journal of proteome research
The emergence of Carbapenem-resistant (CRKP) represents a major public health concern, primarily driven by its ability to evade a wide range of antibiotics. Despite extensive genomic studies, proteomic insights into antibiotic resistance mechanisms ...

Application of MALDI-TOF MS-based peptidome profiling for the identification of Bacillus cereus, Staphylococcus aureus, and Escherichia coli in single and mixed inoculum.

Food chemistry
The detection of mixed-species bacterial samples plays a vital role in ensuring food safety, yet research in this area remains notably limited. This study investigates the integration of MALDI-TOF MS-derived peptidome profiles with artificial intelli...

A generalized platform for artificial intelligence-powered autonomous enzyme engineering.

Nature communications
Proteins are the molecular machines of life with numerous applications in energy, health, and sustainability. However, engineering proteins with desired functions for practical applications remains slow, expensive, and specialist-dependent. Here we r...

Combinatorial discovery of microtopographical landscapes that resist biofilm formation through quorum sensing mediated autolubrication.

Nature communications
Bio-instructive materials that intrinsically inhibit biofilm formation have significant anti-biofouling potential in industrial and healthcare settings. Since bacterial surface attachment is sensitive to surface topography, we experimentally surveyed...